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Read our guarantee »Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Anti-Rac1 antibody
See all Rac1 products (7) ...
Rabbit polyclonal to Rac1
ELISA, WBmore details
Reacts with
Human
Synthetic peptide corresponding to amino acid residues surrounding S71 of human Rac1 conjugated to KLH.
293 cell lysates transiently transfected with the Rac1 gene.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration information loading...
Immunogen affinity purified
ab71513 is purified through a protein A column, followed by peptide affinity purification.
Polyclonal
IgG
Immunology >> Innate Immunity >> TLR Signaling
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Western blot - Rac1 antibody (ab71513)
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Our Abpromise guarantee covers the use of ab71513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/1000
WB: 1/50 - 1/100.Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa).
Ras related C3 botulinum toxin substrate 1 (RAC1) protein is a GTPase which belongs to the RAS superfamily of small GTP binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases.
Cell membrane; lipid anchor; cytoplasmic side
Western blot - Rac1 antibody (ab71513)

All lanes : Anti-Rac1 antibody (ab71513) at 1/50 dilution
Lane 1 : Nontransfected 293 cell lysate
Lane 2 : 293 cell lysate transiently transfected with the Rac1 gene
Lysates/proteins at 2 µg per lane.
Predicted band size : 21 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
ab71513 has not yet been referenced specifically in any publications.
Publishing research using ab71513? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
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All lanes : Anti-Rac1 antibody (ab71513) at 1/50 dilution
Lane 1 : Nontransfected 293 cell lysate
Lane 2 : 293 cell lysate transiently transfected with the Rac1 gene
Lysates/proteins at 2 µg per lane.
Predicted band size : 21 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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