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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab78139? Please let us know so that we can cite the reference in this datasheet
ab78139 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Rac1 antibody (ab78139)
All lanes : Anti-Rac1 antibody (ab78139) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Secondary
Goat anti-Rabbit HRP-conjugated at 1/5000 dilution
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Rac1 antibody (ab78139)
Immunofluorescence analysis of HeLa cells using ab78139 at 1/50 dilution (yellow) in conjunction with a Donkey anti-Rabbit IgG Cy3 conjugated. Hela cells were fixed with 3.7% paraformaldehyde and permeabilized with a non-ionic detergent. The cells are dual stained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rac1 antibody (ab78139)
Immunohistochemistry analysis of paraffin-embedded Human tubular colorectal carcinoma using ab78139 at 1/1000 dilution with an HRP-DAB detection system. Tissue pretreated with citrate pH6.0 antigen retrieval. Immunoreactivity is seen primarily as diffusive plasma membrane staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rac1 antibody (ab78139)
Immunohistochemistry analysis of paraffin-embedded Human prostate carcinoma using ab78139 at 1/1000 dilution with an HRP-DAB detection system. Tissue pretreated with citrate pH6.0 antigen retrieval. Immunoreactivity is seen primarily as diffusive plasma membrane staining with some stromal background staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rac1 antibody (ab78139)
Immunohistochemistry analysis of paraffin-embedded Human squamous cells carcinoma using ab78139 at 1/1000 dilution with an HRP-DAB detection system. Tissue pretreated with citrate pH6.0 antigen retrieval. Immunoreactivity is seen primarily as diffusive nuclear staining pattern.
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