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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - Rac1 antibody (ab78139)
All lanes : Anti-Rac1 antibody (ab78139) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Secondary
Goat anti-Rabbit HRP-conjugated at 1/5000 dilution
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Rac1 antibody (ab78139)
Immunofluorescence analysis of HeLa cells using ab78139 at 1/50 dilution (yellow) in conjunction with a Donkey anti-Rabbit IgG Cy3 conjugated. Hela cells were fixed with 3.7% paraformaldehyde and permeabilized with a non-ionic detergent. The cells are dual stained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rac1 antibody (ab78139)
Immunohistochemistry analysis of paraffin-embedded Human tubular colorectal carcinoma using ab78139 at 1/1000 dilution with an HRP-DAB detection system. Tissue pretreated with citrate pH6.0 antigen retrieval. Immunoreactivity is seen primarily as diffusive plasma membrane staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rac1 antibody (ab78139)
Immunohistochemistry analysis of paraffin-embedded Human prostate carcinoma using ab78139 at 1/1000 dilution with an HRP-DAB detection system. Tissue pretreated with citrate pH6.0 antigen retrieval. Immunoreactivity is seen primarily as diffusive plasma membrane staining with some stromal background staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rac1 antibody (ab78139)
Immunohistochemistry analysis of paraffin-embedded Human squamous cells carcinoma using ab78139 at 1/1000 dilution with an HRP-DAB detection system. Tissue pretreated with citrate pH6.0 antigen retrieval. Immunoreactivity is seen primarily as diffusive nuclear staining pattern.
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