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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Overview
Product name
Anti-Rac1 antibody
See all Rac1 products (7) ...
Description
Rabbit polyclonal to Rac1
Specificity
Recognizes Rac1b specifically. Does not recognize other Rac1 isoforms.
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide from the internal region of Human Rac1b (the immunogen sequence is not present in the other Rac1 isoforms)
Positive control
WB: HeLa and MCF7 cell lysates. IHC=P: Human colorectal, prostate or squamous cells carcinoma. ICC/IF: HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.07% Sodium Azide
Constituents: 30% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab78139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/50.
IHC-P: 1/1000. Antigen retrieval using citrate pH6.0 is recommended.
WB: 1/1000. Detects a band of approximately 22 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Ras related C3 botulinum toxin substrate 1 (RAC1) protein is a GTPase which belongs to the RAS superfamily of small GTP binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases.
Cellular localization
Cell membrane; lipid anchor; cytoplasmic side
Alternative names
- Cell migration inducing gene 5 protein antibody
- Cell migration-inducing gene 5 protein antibody
- MGC111543 antibody
see all
Database links
- Entrez Gene: 5879 Human
- Entrez Gene: 19353 Mouse
- Omim: 602048 Human
- SwissProt: P63000 Human
- SwissProt: P63001 Mouse
- Unigene: 413812 Human
Anti-Rac1 antibody images:
Western blot - Rac1 antibody (ab78139)
All lanes : Anti-Rac1 antibody (ab78139) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Secondary
Goat anti-Rabbit HRP-conjugated at 1/5000 dilution
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Rac1 antibody (ab78139)
Immunofluorescence analysis of HeLa cells using ab78139 at 1/50 dilution (yellow) in conjunction with a Donkey anti-Rabbit IgG Cy3 conjugated. Hela cells were fixed with 3.7% paraformaldehyde and permeabilized with a non-ionic detergent. The cells are dual stained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rac1 antibody (ab78139)
Immunohistochemistry analysis of paraffin-embedded Human tubular colorectal carcinoma using ab78139 at 1/1000 dilution with an HRP-DAB detection system. Tissue pretreated with citrate pH6.0 antigen retrieval. Immunoreactivity is seen primarily as diffusive plasma membrane staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rac1 antibody (ab78139)
Immunohistochemistry analysis of paraffin-embedded Human prostate carcinoma using ab78139 at 1/1000 dilution with an HRP-DAB detection system. Tissue pretreated with citrate pH6.0 antigen retrieval. Immunoreactivity is seen primarily as diffusive plasma membrane staining with some stromal background staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rac1 antibody (ab78139)
Immunohistochemistry analysis of paraffin-embedded Human squamous cells carcinoma using ab78139 at 1/1000 dilution with an HRP-DAB detection system. Tissue pretreated with citrate pH6.0 antigen retrieval. Immunoreactivity is seen primarily as diffusive nuclear staining pattern.
References for Anti-Rac1 antibody (ab78139)
ab78139 has not yet been referenced specifically in any publications.
Publishing research using ab78139? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"