![Western blot - Anti-Rad1 antibody [4126] (ab5363) image](#ab5363_1.jpg)
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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Overview
Product name
Anti-Rad1 antibody [4126]
See all Rad1 products (5) ...
Description
Mouse monoclonal [4126] to Rad1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
N-terminal fragment of human Rad1 purified from E. coli.
Positive control
HeLa whole cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Purified from ascites by protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE.
Clonality
Monoclonal
Clone number
4126
Myeloma
NS1
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
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Western blot - Anti-Rad1 antibody [4126] (ab5363)
![Western blot - Anti-Rad1 antibody [4126] (ab5363) image](/ps/datasheet/Images/5/ab5363/ab5363_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab5363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 28 kDa (predicted molecular weight: 34 kDa).
Target
Function
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
Tissue specificity
Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
Sequence similarities
Belongs to the rad1 family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO60671
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell cycle checkpoint protein Hrad1 antibody
- Cell cycle checkpoint protein Rad 1 A / B antibody
- Cell cycle checkpoint protein RAD1 antibody
see all
Database links
- Entrez Gene: 5810 Human
- Omim: 603153 Human
- SwissProt: O60671 Human
- Unigene: 38114 Human
Anti-Rad1 antibody [4126] images:
References for Anti-Rad1 antibody [4126] (ab5363)
This product has been referenced in:
- Sun TP & Shieh SY Human FEM1B is required for Rad9 recruitment and CHK1 activation in response to replication stress. Oncogene : (2009). WB; Human.Read more (PubMed: 19330022) »
- Doré ASet al. Crystal structure of the rad9-rad1-hus1 DNA damage checkpoint complex--implications for clamp loading and regulation. Mol Cell 34:735-45 (2009). WB; Human.Read more (PubMed: 19446481) »
See all 2 publications for this product
Publishing research using ab5363? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"