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- Rad1 antibody [4126] (ab5363)
![- Rad1 antibody [4126] (ab5363)](/ps/datasheet/images/ab5363_1.jpg)
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Product Name
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Rad1 antibody [4126]
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See all Rad1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [4126] to Rad1
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Immunogen
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N-terminal fragment of human Rad1 purified from E. coli.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 34 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa whole cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
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Relevance
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Human Rad1 is a component of a heterotrimeric PCNA-like complex that also contains the Rad9 and Hus1 proteins. This complex is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Human Rad1 exhibits significant homology to Rad1 from S. pombe, and its expression in yeast rad1 mutants has been shown to partially restore radiation resistance and G2-checkpoint activity. It has also been shown to possess exonuclease activity.
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Rad1 antibody [4126] (ab5363) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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4126
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Myeloma
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NS1
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: PBS
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Purification notes
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Purified from ascites by protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad1 antibody [4126] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rad1 antibody [4126] images, references, products related to ab5363 and other tools.
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Rad1 antibody [4126] images:
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 - Rad1 antibody [4126] (ab5363)
Predicted band size : 34 kDa
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References for Rad1 antibody [4126] (ab5363)
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This product has been used in: (two most recent references)
Sun TP & Shieh SY Human FEM1B is required for Rad9 recruitment and CHK1 activation in response to replication stress. Oncogene : (2009). WB; Human. PubMed: 19330022
Dor AS et al. Crystal structure of the rad9-rad1-hus1 DNA damage checkpoint complex--implications for clamp loading and regulation. Mol Cell 34:735-45 (2009). WB; Human. PubMed: 19446481
If you publish research using ab5363 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Rad1
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Rad1 antibody [4126] - more information
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Customer reviews (feedback) regarding Rad1 antibody [4126] |
Customer FAQs regarding Rad1 antibody [4126] |
Protocols for Rad1 antibody [4126] |
Price and availability of products related to Rad1 antibody [4126] |
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Rad1 antibody [4126] - related products:
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Rad1 antibody [4126] - other tools:
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Contact Abcam with a Technical Enquiry about Rad1 antibody [4126] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![- Rad1 antibody [4126] (ab5363)](/index.html?datasheet=5363#ab5363_1.jpg)
