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Product Name
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Rad1 antibody
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See all Rad1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rad1
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Immunogen
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Synthetic peptide: PLLTQQIQDEDDQYSLVA conjugated to KLH via a C-terminal Cysteine residue linker, corresponding to N terminal amino acids 2 - 19 of Human Rad1
BLAST 'PLLTQQIQDEDDQYSLVA' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: 5-10 ul immunoprecipitates RAD1 from HeLa cell lysates.
WB: 1/250 - 1/500. Detects a band of approximately 30 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysates; Extracts of HL-60 cells
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
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Relevance
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Human Rad1 is a component of a heterotrimeric PCNA-like complex that also contains the Rad9 and Hus1 proteins. This complex is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Human Rad1 exhibits significant homology to Rad1 from S. pombe, and its expression in yeast rad1 mutants has been shown to partially restore radiation resistance and G2-checkpoint activity. It has also been shown to possess exonuclease activity.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum protein
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Form
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Liquid
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Concentration
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9.100 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Rad1
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Rad1 antibody - more information
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