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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Overview
Product name
Anti-Rad1 antibody
See all Rad1 products (5) ...
Description
Rabbit polyclonal to Rad1
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: PLLTQQIQDEDDQYSLVA conjugated to KLH via a C-terminal Cysteine residue linker, corresponding to N terminal amino acids 2 - 19 of Human Rad1
PLLTQQIQDE DDQYSLVA
Positive control
HeLa cell lysates; Extracts of HL-60 cells
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum protein
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
WB
Application notes
IP: 5-10 ul immunoprecipitates RAD1 from HeLa cell lysates.
WB: 1/250 - 1/500. Detects a band of approximately 30 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
Tissue specificity
Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
Sequence similarities
Belongs to the rad1 family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO60671
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell cycle checkpoint protein Hrad1 antibody
- Cell cycle checkpoint protein Rad 1 A / B antibody
- Cell cycle checkpoint protein RAD1 antibody
see all
Database links
- Entrez Gene: 5810 Human
- Omim: 603153 Human
- SwissProt: O60671 Human
- Unigene: 38114 Human
References for Anti-Rad1 antibody (ab50310)
ab50310 has not yet been referenced specifically in any publications.
Publishing research using ab50310? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"