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Anti-Rad1 antibody (ab76830) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
developed using the ECL technique
Predicted band size : 32 kDa
Observed band size : 32 kDa
Anti-Rad1 antibody (ab76830) at 5 µg/ml + HeLa cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 32 kDa
Observed band size : 32 kDa
All lanes : Anti-Rad1 antibody (ab76830) at 5 µg/ml
Lane 1 : Rad1 transfected 293T cell line
Lane 2 : Non transfected 293T cell line
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 32 kDa
Observed band size : 32 kDa
Immunohistochemical analysis of formalin-fixed paraffin-embedded human lateral ventricle wall using ab 76830 at 3 µg/ml.
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