|
|
|
|
Western blot - Rad1 antibody (ab76830)
(enlarge)
|
|
Western blot - Rad1 antibody (ab76830)
(enlarge)
|
|
Western blot - Rad1 antibody (ab76830)
(enlarge)
|
|
|
|
|
Product Name
|
Rad1 antibody
|
| |
See all Rad1 antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal to Rad1
|
|
Immunogen
|
Recombinant tagged fragment: MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL , corresponding to amino acids 1-90 of Human Rad1.
BLAST 'MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL ' with  or 
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
IHC-P, WB, sELISA
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IHC-P: Use at a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
sELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
HeLa cell lysate.
RAD1 transfected 293T cell line.
Human lateral ventricle wall tissue.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
|
|
Relevance
|
Human Rad1 is a component of a heterotrimeric PCNA-like complex that also contains the Rad9 and Hus1 proteins. This complex is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Human Rad1 exhibits significant homology to Rad1 from S. pombe, and its expression in yeast rad1 mutants has been shown to partially restore radiation resistance and G2-checkpoint activity. It has also been shown to possess exonuclease activity.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Isotype
|
IgG3
|
|
Light chain type
|
kappa
|
|
Purity
|
Protein A purified
|
|
Storage buffer
|
Preservative: None
Constituents: 1X PBS, pH 7.2
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Rad1 antibody images, references, products related to ab76830 and other tools.
|
| |
Rad1 antibody images:
|
 Western blot - Rad1 antibody (ab76830)
Rad1 antibody (ab76830) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
developed using the ECL technique
Predicted band size : 32 kDa
Observed band size : 32 kDa
|
| |
Western blot - Rad1 antibody (ab76830)
Rad1 antibody (ab76830) at 5 µg/ml + HeLa cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 32 kDa
Observed band size : 32 kDa
|
| |
Western blot - Rad1 antibody (ab76830)
All lanes : Rad1 antibody (ab76830) at 5 µg/ml
Lane 1 : Rad1 transfected 293T cell line
Lane 2 : Non transfected 293T cell line
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 32 kDa
Observed band size : 32 kDa
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rad1 antibody (ab76830)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human lateral ventricle wall using ab 76830 at 3 µg/ml.
|
| |
| |
|
Search PubMed (MEDLINE) for references to Rad1
|
| |
Rad1 antibody - more information
|
Customer reviews (feedback) regarding Rad1 antibody |
Customer FAQs regarding Rad1 antibody |
Protocols for Rad1 antibody |
Price and availability of products related to Rad1 antibody |
| |
Rad1 antibody - related products:
|
|
|
| |
Rad1 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Rad1 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
