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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Overview
Product name
Anti-Rad1 antibody
See all Rad1 products (5) ...
Description
Mouse monoclonal to Rad1
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Recombinant tagged fragment: MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL , corresponding to amino acids 1-90 of Human Rad1.
MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL
Positive control
HeLa cell lysate. RAD1 transfected 293T cell line. Human lateral ventricle wall tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG3
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IHC-P: Use a concentration of 3 µg/mlPerform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Target
Function
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
Tissue specificity
Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
Sequence similarities
Belongs to the rad1 family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO60671
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell cycle checkpoint protein Hrad1 antibody
- Cell cycle checkpoint protein Rad 1 A / B antibody
- Cell cycle checkpoint protein RAD1 antibody
see all
Database links
- Entrez Gene: 5810 Human
- Entrez Gene: 19355 Mouse
- Omim: 603153 Human
- SwissProt: O60671 Human
- SwissProt: Q9QWZ1 Mouse
- Unigene: 38114 Human
- Unigene: 38376 Mouse
Anti-Rad1 antibody images:
Western blot - Rad1 antibody (ab76830)
Anti-Rad1 antibody (ab76830) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
developed using the ECL technique
Predicted band size : 32 kDa
Observed band size : 32 kDa
Western blot - Rad1 antibody (ab76830)
Anti-Rad1 antibody (ab76830) at 5 µg/ml + HeLa cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 32 kDa
Observed band size : 32 kDa
Western blot - Rad1 antibody (ab76830)
All lanes : Anti-Rad1 antibody (ab76830) at 5 µg/ml
Lane 1 : Rad1 transfected 293T cell line
Lane 2 : Non transfected 293T cell line
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 32 kDa
Observed band size : 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rad1 antibody (ab76830)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human lateral ventricle wall using ab 76830 at 3 µg/ml.
References for Anti-Rad1 antibody (ab76830)
ab76830 has not yet been referenced specifically in any publications.
Publishing research using ab76830? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"