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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
Overview
Product name
Anti-Rad17 (phospho S635) antibody
See all Rad17 products (14) ...
Description
Rabbit polyclonal to Rad17 (phospho S635)
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Cross reactivity
Predicted to work with
Human, Orangutan
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human Rad17, phosphorylated at S635 and phosphorylated at S635.
(Peptide available as ab28625.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
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ELISA - Rad17 (phospho S635) antibody (ab26335)
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Applications
Application notes
This antibody gave a positive result in ELISA against the immunizing peptide (ab28625). It gave a negative result in ELISA against the non-modified equivalent peptide (ab28626). This indicates that it is specific for the modified peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
Tissue specificity
Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
Sequence similarities
Belongs to the rad17/RAD24 family.
Post-translational
modifications
Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
Cellular localization
Nucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
Target information above from: UniProt accessionO75943
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CCYC antibody
- Cell cycle checkpoint protein antibody
- Cell cycle checkpoint protein RAD17 antibody
see all
Database links
- Entrez Gene: 5884 Human
- Omim: 603139 Human
- SwissProt: O75943 Human
- Unigene: 16184 Human
Anti-Rad17 (phospho S635) antibody images:
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
ELISA - Rad17 (phospho S635) antibody (ab26335)
This antibody gave a positive result in ELISA against the immunizing peptide (ab28625). It gave a negative result in ELISA against the non-modified equivalent peptide (ab28626). This indicates that it is specific for the modified peptide.
References for Anti-Rad17 (phospho S635) antibody (ab26335)
ab26335 has not yet been referenced specifically in any publications.
Publishing research using ab26335? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"