Rad17 (phospho S645) antibody (ab3620)

Product Name 

Rad17 (phospho S645) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Rad17 (phospho S645)

Immunogen 

Synthetic phospho peptide (Human) - which represents a portion of human Rad 17around serine 645.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: 1/500 - 1/5000. Detects a band of approximately 85-88 kDa (predicted molecular weight: 77 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa whole cell lysate treated with 10 Gy IR.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition

Relevance 

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe Rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA damage induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Tris-citrate/phosphate, pH 7-8, 0.1% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Antibodies that were not phospho-specific were removedby solid phase absorption.
Antibodies specific for Rad 17pSer 645 were affinity purified using the phosphopeptideimmobilized on solid support.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

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References for Rad17 (phospho S645) antibody (ab3620)

  This product has been used in:

Jurvansuu J et al. Viral transport of DNA damage that mimics a stalled replication fork. J Virol 79:569-80 (2005). PubMed: 15596849

If you publish research using ab3620 please let us know so that we can cite the reference on this datasheet.

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