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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Overview
Product name
Anti-Rad17 (phospho S645) antibody
See all Rad17 products (14) ...
Description
Rabbit polyclonal to Rad17 (phospho S645)
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic phospho peptide (Human) - which represents a portion of human Rad 17around serine 645.
Positive control
HeLa whole cell lysate treated with 10 Gy IR.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Antibodies that were not phospho-specific were removedby solid phase absorption. Antibodies specific for Rad 17pSer 645 were affinity purified using the phosphopeptideimmobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
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Western blot - Rad17 (phospho S645) antibody (ab3620)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/5000.Detects a band of approximately 85-88 kDa (predicted molecular weight: 77 kDa).
ICC/IF: Use at an assay dependent dilution. (PubMed: 20368419)
Target
Function
Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
Tissue specificity
Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
Sequence similarities
Belongs to the rad17/RAD24 family.
Post-translational
modifications
Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
Cellular localization
Nucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
Target information above from: UniProt accessionO75943
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CCYC antibody
- Cell cycle checkpoint protein antibody
- Cell cycle checkpoint protein RAD17 antibody
see all
Database links
- Entrez Gene: 5884 Human
- Omim: 603139 Human
- SwissProt: O75943 Human
- Unigene: 16184 Human
Anti-Rad17 (phospho S645) antibody images:
Western blot - Rad17 (phospho S645) antibody (ab3620)
Predicted band size : 77 kDa
Sample: Whole Cell Lysate (50
Rabbit anti-Rad 17 pSer645 (ab3620) used at 1:1,000 dilution
References for Anti-Rad17 (phospho S645) antibody (ab3620)
This product has been referenced in:
- Kumar Aet al. Nuclear phosphoinositide 3-kinase beta controls double-strand break DNA repair. Proc Natl Acad Sci U S A 107:7491-6 (2010). WB, ICC/IF; Mouse.Read more (PubMed: 20368419) »
- Jurvansuu Jet al. Viral transport of DNA damage that mimics a stalled replication fork. J Virol 79:569-80 (2005).Read more (PubMed: 15596849) »
See all 2 publications for this product
Publishing research using ab3620? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"