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Immunocytochemistry/ Immunofluorescence - Rad21 antibody [CM110-2C10] (ab16473)
![Immunocytochemistry/ Immunofluorescence - Rad21 antibody [CM110-2C10] (ab16473)](/ps/datasheet/images/ab16473_1.jpg)
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Product Name
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Rad21 antibody [CM110-2C10]
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See all Rad21 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [CM110-2C10] to Rad21
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Immunogen
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Synthetic peptide: ASGGDQD QEERRWNKRT QQMLHGLQRA LAKTGAESIS LLELCRNTNR KQAAAKFYSF, corresponding to C terminal amino acids 544-631 of Human Rad21.
BLAST 'ASGGDQD QE ERRWNKRT Q QMLHGLQRA LAKTGAESIS LLELCRNTN R KQAAAKFY ' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Xl, Zfsh
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Used at a concentration of 1/100 for 16 hrs on HeLa cells (see Abreview submitted by Kirk McManus).
WB: 1/500. Predicted molecular weight: 72 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa whole cell lysate.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cancer >> Cell cycle >> Cell division
Chromatin and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Chromatid Cohesion
Chromatin and Nuclear Signaling >> Chromosome Structure >> Cohesins
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
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Relevance
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The protein encoded by the Rad21 gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase.
RAD21 also plays a role in apoptosis, via its cleavage by caspase 3 or caspase 7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. Recent work has shown that the RAD21 has a role in the invasion and metastasis of cancer cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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CM110-2C10
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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From ascites using protein G.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad21 antibody [CM110-2C10] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rad21 antibody [CM110-2C10] images, references, products related to ab16473 and other tools.
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Rad21 antibody [CM110-2C10] images:
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 Immunocytochemistry/ Immunofluorescence - Rad21 antibody [CM110-2C10] (ab16473)
ab16473 at a 1/100 dilution staining aynchronous HeLa cells by ICC/IF. The cells were paraformaldehyde fixed and incubated with the antibody overnight. Bound antibody was detected using a Cy3 conjugated goat anti-mouse antibody. Nuclei were visualised using DAPI stain. ab16473 gives a very faint staining pattern that is enriched within the nucleus. This image is courtesy of an Abreview submitted by Kirk McManus.
See Abreview
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Search PubMed (MEDLINE) for references to Rad21
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Rad21 antibody [CM110-2C10] - more information
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Customer reviews (feedback) regarding Rad21 antibody [CM110-2C10] |
Customer FAQs regarding Rad21 antibody [CM110-2C10] |
Protocols for Rad21 antibody [CM110-2C10] |
Price and availability of products related to Rad21 antibody [CM110-2C10] |
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Rad21 antibody [CM110-2C10] - related products:
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Rad21 antibody [CM110-2C10] - other tools:
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Contact Abcam with a Technical Enquiry about Rad21 antibody [CM110-2C10] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - Rad21 antibody [CM110-2C10] (ab16473)](/index.html?datasheet=16473#ab16473_1.jpg)
