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Western blot - Rad21 antibody (ab42522)
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Immunocytochemistry/ Immunofluorescence - Rad21 antibody (ab42522)
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Product Name
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Rad21 antibody
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See all Rad21 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rad21
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Rad21. (Peptide available as ab42521.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Chk, Cow
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 75 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line),
HEK293 (Human embryonic kidney cell line),
A431 (Human epithelial carcinoma cell line),
Jurkat (Human T cell lymphoblast-like cell line).
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cancer >> Cell cycle >> Cell division
Chromatin and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Chromatid Cohesion
Chromatin and Nuclear Signaling >> Chromosome Structure >> Cohesins
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
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Relevance
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The protein encoded by the Rad21 gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase.
RAD21 also plays a role in apoptosis, via its cleavage by caspase 3 or caspase 7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. Recent work has shown that the RAD21 has a role in the invasion and metastasis of cancer cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad21 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rad21 antibody images, references, products related to ab42522 and other tools.
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Rad21 antibody images:
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 Western blot - Rad21 antibody (ab42522)
All lanes : Rad21 antibody (ab42522) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 75 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Although the predicted band size is 75kDa based on Swiss-prot data, a band of 120kDa has been previously observed. Mol Cell Biol. 2002 Dec;22(23):8267-77 PMID: 12417729. The band seen at 100kDa is also consistent with the banding pattern observed for other commercially available antibodies to Rad21.
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Immunocytochemistry/ Immunofluorescence - Rad21 antibody (ab42522)
ICC/IF image of ab42522 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab42522, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Search PubMed (MEDLINE) for references to Rad21
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Rad21 antibody - more information
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Customer reviews (feedback) regarding Rad21 antibody |
Customer FAQs regarding Rad21 antibody |
Protocols for Rad21 antibody |
Price and availability of products related to Rad21 antibody |
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Rad21 antibody - related products:
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Rad21 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Rad21 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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