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Products:Epigenetics and Nuclear Signaling >> Chromosome Structure >> Telomeres
Overview
Product name
Anti-Rad50 antibody
See all Rad50 products (8) ...
Description
Rabbit polyclonal to Rad50
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide (Human) - which represented a portion of human Rad 50 encoded in partby exons 2 and 3.
Positive control
HeLa cell nuclear extract.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Immunoprecipitation - Rad50 antibody (ab3622)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab3622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Detects a band of approximately 157 kDa (predicted molecular weight: 150 kDa).
IP: Use a concentration of 1 - 4 µg/ml.
Target
Function
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
Tissue specificity
Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
Involvement in disease
Defects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent.
Sequence similarities
Belongs to the SMC family. RAD50 subfamily.
Contains 1 zinc-hook domain.
Domain
The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus. Chromosome > telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
Target information above from: UniProt accessionQ92878
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA repair protein RAD50 antibody
- hRad50 antibody
- hsRAD50 antibody
see all
Database links
- Entrez Gene: 10111 Human
- Entrez Gene: 19360 Mouse
- Entrez Gene: 64012 Rat
- Omim: 604040 Human
- SwissProt: Q92878 Human
- SwissProt: P70388 Mouse
- SwissProt: Q9JIL8 Rat
- Unigene: 633509 Human
see all
Anti-Rad50 antibody images:
Immunoprecipitation - Rad50 antibody (ab3622)
Lanes:
1. IP from 1mg extract with 1.25g Rabbit anti-Rad 50 (exon 2-3) (ab3622)
2. IP from 1mg extract with 1.25
Sample: Nuclear extract from Hela cells
for Western blot, affinity purified Rabbit anti-Rad 50 (ab3622) used at 1:4000 dilution
References for Anti-Rad50 antibody (ab3622)
This product has been referenced in:
- Tommiska Jet al. Evaluation of RAD50 in familial breast cancer predisposition. Int J Cancer 118:2911-6 (2006).Read more (PubMed: 16385572) »
See 1 publication for this product
Publishing research using ab3622? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"