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Western blot - Rad50 antibody (ab3623)
(enlarge)
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Product Name
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Rad50 antibody
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See all Rad50 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rad50
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Immunogen
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Synthetic peptide (Human) - which represents a portion of human Rad 50 encoded in part by exons 13 and 14.
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Reacts with
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Hu
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Predicted to react
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Ms, Rat
(Due to sequence homology)
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at a concentration of 1 - 4 µg/ml.
WB: 1/1000 - 1/5000. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell nuclear extract.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Non Homol. End Joining
Chromatin and Nuclear Signaling >> Chromosome Structure >> Telomeres
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Relevance
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Rad50 is a component of the MRN complex, which plays a critical role in double-strand break repair (DSB), DNA recombination, maintenance of telomere integrity, and meiosis. The MRE11A component of this complex has both single-strand endonuclease and double strand-specific 3'-5' exonuclease activities. Rad50 is postulated to bind to DNA ends and hold them in close proximity, thus facilitating searches for regions of sequence homology, and also possibly stimulating the activity of DNA ligases and/or restricting MRE11A activity. Rad50 is phosphorylated upon DNA damage, probably by ATM or ATR.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Tris-citrate/phosphate, pH 7-8, 0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad50 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rad50 antibody images, references, products related to ab3623 and other tools.
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Rad50 antibody images:
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 Western blot - Rad50 antibody (ab3623)
Predicted band size : 160 kDa
Sample: Nuclear extract (50µg) from Hela cells Affinity purified Rabbit anti-Rad 50 (ab3623) used at 1:4,000 dilution
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Search PubMed (MEDLINE) for references to Rad50
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Rad50 antibody - more information
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Customer reviews (feedback) regarding Rad50 antibody |
Customer FAQs regarding Rad50 antibody |
Protocols for Rad50 antibody |
Price and availability of products related to Rad50 antibody |
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Rad50 antibody - related products:
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Rad50 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Rad50 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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