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Product Name
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Rad50 antibody
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See all Rad50 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rad50
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Immunogen
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Fusion protein with C-terminus of mRad50 (604 amino acids) (Mouse).
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Reacts with
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Hu, Ms
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Specificity
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This antibody is specific for Rad50.
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use 5 µl for 3-4 X 106 cells.
WB: 1/2000. Predicted molecular weight: 153 kDa in human cells.
Is unsuitable for ICC/IF.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Non Homol. End Joining
Chromatin and Nuclear Signaling >> Chromosome Structure >> Telomeres
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Relevance
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Rad50 is a component of the MRN complex, which plays a critical role in double-strand break repair (DSB), DNA recombination, maintenance of telomere integrity, and meiosis. The MRE11A component of this complex has both single-strand endonuclease and double strand-specific 3'-5' exonuclease activities. Rad50 is postulated to bind to DNA ends and hold them in close proximity, thus facilitating searches for regions of sequence homology, and also possibly stimulating the activity of DNA ligases and/or restricting MRE11A activity. Rad50 is phosphorylated upon DNA damage, probably by ATM or ATR.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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serum with 0.02% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad50 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Rad50 antibody (ab499)
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This product has been used in: (two most recent references)
Bentley J et al. DNA double strand break repair in human bladder cancer is error prone and involves microhomology-associated end-joining. Nucleic Acids Res 32:5249-59 (2004). PubMed: 15466592
Cerosaletti KM & Concannon P Nibrin Forkhead-associated Domain and Breast Cancer C-terminal Domain Are Both Required for Nuclear Focus Formation and Phosphorylation. J Biol Chem 278:21944-51 (2003). PubMed: 12679336
See all 3 publication references for this product.
If you publish research using ab499 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Rad50
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Rad50 antibody - more information
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Customer reviews (feedback) regarding Rad50 antibody |
Customer FAQs regarding Rad50 antibody |
Protocols for Rad50 antibody |
Price and availability of products related to Rad50 antibody |
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Rad50 antibody - related products:
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Rad50 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Rad50 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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