Overview
- Product nameRad51 293T Transfected Lysate - (positive control)
- General notesab94044 is a 293T cell transfected lysate in which Human Rad51 has been transiently over-expressed using a pCMV-Rad51 plasmid. The lysate is provided in 1X Sample Buffer.
Note: For more detailed how the transfected lysate was prepared view preparation notes
- Tested applicationsWB
more details
Properties
- FormLiquid
- Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
- Storage bufferpH: 6.80
Constituent: 100% 1x Sample Buffer
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Concentration information loading...
- PurityWhole Cell Lysate
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Research Areas
BackgroundDisease: Breast cancer (BC) [MIM:114480]: A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Mirror movements 2 (MRMV2) [MIM:614508]: A disorder characterized by contralateral involuntary movements that mirror voluntary ones. While mirror movements are occasionally found in young children, persistence beyond the age of 10 is abnormal. Mirror movements occur more commonly in the upper extremities. Note=The disease is caused by mutations affecting the gene represented in this entry.
Domain: The nuclear localization may reside in the C-terminus (between 259 and 339 AA).
Function: Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
PTM: Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
Similarity: Belongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain.
Tissue specificity: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
Applications
Our Abpromise guarantee covers the use of
ab94044
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Rad51 293T Transfected Lysate - (positive control) images
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SDS-PAGE - Rad51 293T Transfected Lysate - (positive control) (ab94044)
ab94044 at 15µg/lane on an SDS-PAGE gel.
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Western blot - Rad51 293T Transfected Lysate - (positive control) (ab94044)
All lanes : Anti-Rad51 antibody (ab88572) at 1/500 dilution
Lane 1 : ab94044
Lane 2 : non transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
non-Abcam, Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
References for Rad51 293T Transfected Lysate - (positive control) (ab94044)
ab94044
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
