Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
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ab213 has been referenced in 13 publications.
Publishing research using ab213? Please let us know so that we can cite the reference in this datasheet
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ab213 staining Rad51 in HeLa cells by Immunocytochemistry/ Immunoflourescence. Cells were PFA-fixed and permeabilized in methanol for 30 minutes at -20ºC prior to blocking in 20% FCS for 30 minutes at room temperature. The primary antibody was diluted 1/500 with the blocking buffer and incubated with the sample for 1 hour. The secondary antibody was a Alexa Fluor 594®-conjugated Goat anti-Mouse polyclonal, diluted 1/1000.
Cells were irradiated with 10 Gy and fixed 2 hours later. DAPI was used for the nuclear counterstain.
This image is courtesy of an Abreview by Ioannis Gavvodis.
Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution + lysate prepared from murine NIH3T3 cells at 20 µg
Secondary
HRP conjugated anti-mouse IgG at 1/5000 dilution
developed using the ECL technique
Predicted band size : 37 kDa
Observed band size : 42,45 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
Image courtesy of an anonymous Abreview.
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