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Product Name
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Rad51 antibody [14B4]
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See all Rad51 antibodies (15)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [14B4] to Rad51
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Immunogen
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Recombinant full length protein, corresponding to amino acids 1-138 of Human Rad51
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Reacts with
(species key)
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Hu
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Specificity
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Specific for human Rad51.
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/200. This antibody worked in HeLa cells (see S. Hussain et al., Hum Mol Genet 13:1241-8 (2004)), however we have also received reports stating that this antibody failed to work in SK-N-SH cells treated with bleomycin. We would welcome further customer feedback about ab213 used in ICC/IF.
IP: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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T24 Bladder carcinoma cells and recombinant fusion protein.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
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Relevance
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Rad51 is a recombinase that is essential for homologous recombination and double-strand break repair. It binds to single and double-stranded DNA, and has DNA-dependent ATPase activity. Rad51 unwinds duplex DNA and forms helical nucleoprotein filaments in a manner that is controlled through the action of DNA helicases that counteract nucleofilament formation. Rad51 interacts with BRCA1 and BRCA2, and also plays a role in p53-mediated signalling. Interaction of Rad51 with Chk1 kinase may occur in response to Chk1 phosphorylation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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14B4
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Myeloma
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NS1
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Isotype
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IgG2b
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Light chain type
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kappa
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Purity
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Immunogen affinity purified
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Storage buffer
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Constituents: PBS, pH 7.4
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Purification notes
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Purified from hybridoma cell culture supernatant by protein G chromotography.
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Form
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Liquid
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Concentration
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Batch dependent within range: 1.00 - 2.00 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad51 antibody [14B4] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Rad51 antibody [14B4] (ab213)
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This product has been used in: (two most recent references)
Santarosa M et al. Premature senescence is a major response to DNA cross-linking agents in BRCA1-defective cells: implication for tailored treatments of BRCA1 mutation carriers. Mol Cancer Ther 8:844-54 (2009). ICC/IF; Human. PubMed: 19372557
Thorslund T et al. Interactions between human BRCA2 protein and the meiosis-specific recombinase DMC1. EMBO J 26:2915-22 (2007). WB; Human. PubMed: 17541404
See all 10 publication references for this product.
If you publish research using ab213 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Rad51
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Rad51 antibody [14B4] - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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