|
|
|
|
|
Product Name
|
Rad51 antibody [51RAD01]
|
| |
See all Rad51 antibodies (15)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [51RAD01] to Rad51
|
|
Immunogen
|
BALB/C mice were injected with recombinant Rad51 protein.
|
|
Reacts with
(species key)
|
Hu, Ms, Rat
|
|
Specificity
|
This antibody is specific to a 37-43 kDa protein known as Rad51.
|
|
Tested applications
(see key)
|
ICC/IF, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: 1/10. PMID 18663141.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 37 kDa.
This antibody gives inconsistent results on tissue sections in IHC staining and we cannot guarantee it for IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Testis
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
|
|
Relevance
|
Rad51 is a recombinase that is essential for homologous recombination and double-strand break repair. It binds to single and double-stranded DNA, and has DNA-dependent ATPase activity. Rad51 unwinds duplex DNA and forms helical nucleoprotein filaments in a manner that is controlled through the action of DNA helicases that counteract nucleofilament formation. Rad51 interacts with BRCA1 and BRCA2, and also plays a role in p53-mediated signalling. Interaction of Rad51 with Chk1 kinase may occur in response to Chk1 phosphorylation.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
51RAD01
|
|
Isotype
|
IgG1
|
|
Light chain type
|
kappa
|
|
Purity
|
IgG fraction
|
|
Storage buffer
|
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.100 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad51 antibody [51RAD01] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
References for Rad51 antibody [51RAD01] (ab1837)
|
|
This product has been used in: (two most recent references)
Zheng L et al. MRE11 complex links RECQ5 helicase to sites of DNA damage. Nucleic Acids Res 37:2645-57 (2009). WB; Human. PubMed: 19270065
Chan KL et al. Replication stress induces sister-chromatid bridging at fragile site loci in mitosis. Nat Cell Biol 11:753-60 (2009). WB; Human. PubMed: 19465922
See all 8 publication references for this product.
If you publish research using ab1837 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to Rad51
|
| |
Rad51 antibody [51RAD01] - more information
|
Customer reviews (feedback) regarding Rad51 antibody [51RAD01] |
Customer FAQs regarding Rad51 antibody [51RAD01] |
Protocols for Rad51 antibody [51RAD01] |
Price and availability of products related to Rad51 antibody [51RAD01] |
| |
Rad51 antibody [51RAD01] - related products:
|
|
|
| |
Rad51 antibody [51RAD01] - other tools:
|
Contact Abcam with a Technical Enquiry about Rad51 antibody [51RAD01] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
