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Anti-Rad51 antibody (ab46981)

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1 question for ab46981

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Question 1

Tuesday 22-November-2011

Dear Sirs,

I would like to order anti-RAD51 antibodies from you company (through

Sellex here in Brazil). A coleague used the ab46981 and it work against

Leishmania.

However, looking your catalog I found the ab63801, that was used to

labels focci induced by DNA damage.

My question is?

1- Are these two just the same antibody with different purification

steps (the first is whole serum and the second protein A purified) as

the same immunogen was used?

2- They both work binding to focci? (in the catalog figure you have the

46981 labels extensively the cell cytosol).

ANSWER:

  

Thank you for your enquiry. Regarding question 1: No, these are two different rabbit anti-Rad51 antibodies. Regarding question 2: Both antibodies bind Rad51 and should label foci of cells induced with DNA damage. The ICC image on the datasheet of ab46981 may be showing heavy staining on the edges of the nuclei, rather than cytoplasmic staining. Although I can't confirm that. I hope this is helpful. Please contact me again if you have any further questions.

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