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Western blot - Rad51 antibody (ab63801)
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Immunocytochemistry/ Immunofluorescence - Rad51 antibody (ab63801)
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Product Name
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Rad51 antibody
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See all Rad51 antibodies (15)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rad51
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Immunogen
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Purified recombinant full length protein (Human)
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Reacts with
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Hu, Ms, Chk
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/100.
IP: Use at an assay dependent dilution.
WB: 1/2000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell extract. Normal human diploid cells.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
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Relevance
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Rad51 is a recombinase that is essential for homologous recombination and double-strand break repair. It binds to single and double-stranded DNA, and has DNA-dependent ATPase activity. Rad51 unwinds duplex DNA and forms helical nucleoprotein filaments in a manner that is controlled through the action of DNA helicases that counteract nucleofilament formation. Rad51 interacts with BRCA1 and BRCA2, and also plays a role in p53-mediated signalling. Interaction of Rad51 with Chk1 kinase may occur in response to Chk1 phosphorylation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: Whole serum Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad51 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rad51 antibody images, references, products related to ab63801 and other tools.
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Rad51 antibody images:
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 Western blot - Rad51 antibody (ab63801)
All lanes : Rad51 antibody (ab63801) at 1/2000 dilution
Lane 1 : Molecular weight markers
Lane 2 : HeLa cell extract
Predicted band size : 37 kDa
Observed band size : 37 kDa
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Immunocytochemistry/ Immunofluorescence - Rad51 antibody (ab63801)
Immunofluorescent detection of Rad51 foci formation induced by DNA damage. Normal human diploid cells were irradiated by X-ray (0.5 Gly) and after incubation of 6 hr, the cells were fixed and immuno-stained by using ab63801
(x100 dilution) as the primary antibody and Alexa594 labeled anti-rabbit antibody as the secondary antibody.
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Search PubMed (MEDLINE) for references to Rad51
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Rad51 antibody - more information
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Customer reviews (feedback) regarding Rad51 antibody |
Customer FAQs regarding Rad51 antibody |
Protocols for Rad51 antibody |
Price and availability of products related to Rad51 antibody |
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Rad51 antibody - related products:
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Rad51 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Rad51 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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