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Anti-Rad51 antibody (ab84304)


  • Product nameAnti-Rad51 antibody
    See all Rad51 primary antibodies
  • Description
    Rabbit polyclonal to Rad51
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Chicken, Cow, Dog, ZebrafishDoes not react with: Arabidopsis thaliana
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Rad51.

    (Peptide available as ab92664.)

  • Positive control
    • WB: Human testis tissue lysate and PC12 whole cell, HeLa whole cell, and HeLa nuclear lysates. ICC/IF: HepG2 cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab84304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).Can be blocked with Human Rad51 peptide (ab92664).


  • FunctionParticipates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
  • Tissue specificityHighly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
  • Involvement in diseaseBreast cancer
    Mirror movements 2
  • Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
    Contains 1 HhH domain.
  • DomainThe nuclear localization may reside in the C-terminus (between 259 and 339 AA).
  • Post-translational
    Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRCA1/BRCA2 containing complex, subunit 5 antibody
    • BRCC 5 antibody
    • BRCC5 antibody
    • DNA repair protein RAD51 homolog 1 antibody
    • DNA repair protein rhp51 antibody
    • hRAD51 antibody
    • HsRAD51 antibody
    • HsT16930 antibody
    • MRMV2 antibody
    • Rad 51 antibody
    • RAD51 antibody
    • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
    • RAD51 homolog A antibody
    • RAD51 homolog antibody
    • RAD51 recombinase antibody
    • RAD51, S. cerevisiae, homolog of antibody
    • RAD51_HUMAN antibody
    • RAD51A antibody
    • RECA antibody
    • RecA like protein antibody
    • RecA, E. coli, homolog of antibody
    • Recombination protein A antibody
    see all

Anti-Rad51 antibody images

  • ICC/IF image of ab84304 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84304, 5ug/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Rad51 antibody (ab84304) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Anti-Rad51 antibody (ab84304) at 1 µg/ml + PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References for Anti-Rad51 antibody (ab84304)

ab84304 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 900 µg
Gel Running Conditions Reduced Denaturing (12%, Laemmli)
Sample Arabidopsis thaliana Tissue lysate - whole (closed flower buds)
Specification closed flower buds
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Natasha Elina

Verified customer

Submitted Mar 04 2014

Thank you for contacting us and for providing this essential information.

As the identity and positives for these species are very similar I would be able to provide you with Abreview discounts should you want to test a product. When choosin...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS

Dr. Kirk McManus

Verified customer

Submitted Jun 16 2010