Anti-Rad51 antibody (ab84304)

Overview

• Product nameAnti-Rad51 antibodySee all Rad51 primary antibodies ...
• Description
  Rabbit polyclonal to Rad51
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Rat, Human
  Predicted to work with: Mouse, Rabbit, Chicken, Cow, Dog, Zebrafish
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Rad51.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab92664.)

• Positive controlThis antibody gave a positive signal in human testis tissue lysate and the following cell lysates: PC12 whole cell; HeLa whole cell; HeLa nuclear.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Homologous Recomb.

Applications

Target

• FunctionParticipates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
• Tissue specificityHighly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
• Involvement in diseaseBreast cancer (BC) [MIM:114480]: A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
  Mirror movements 2 (MRMV2) [MIM:614508]: A disorder characterized by contralateral involuntary movements that mirror voluntary ones. While mirror movements are occasionally found in young children, persistence beyond the age of 10 is abnormal. Mirror movements occur more commonly in the upper extremities. Note=The disease is caused by mutations affecting the gene represented in this entry.
• Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
  Contains 1 HhH domain.
• DomainThe nuclear localization may reside in the C-terminus (between 259 and 339 AA).
• Post-translational
  modificationsPhosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
• Cellular localizationNucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion matrix. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels.

Target information above from: UniProt accession Q06609 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 396086 Chicken
  • Entrez Gene: 514749 Cow
  • Entrez Gene: 403568 Dog
  • Entrez Gene: 5888 Human
  • Entrez Gene: 19361 Mouse
  • Entrez Gene: 100008661 Rabbit
  • Entrez Gene: 499870 Rat
  • Entrez Gene: 406487 Zebrafish

  • Omim: 179617 Human
  • SwissProt: P37383 Chicken
  • SwissProt: Q2KJ94 Cow
  • SwissProt: Q8MKI8 Dog
  • SwissProt: Q06609 Human
  • SwissProt: Q08297 Mouse
  • SwissProt: O77507 Rabbit
  • Unigene: 631709 Human
  • Unigene: 330492 Mouse
  • Unigene: 20474 Rat

  see all

• Alternative names
  BRCA1/BRCA2 containing complex, subunit 5 antibodyBRCC 5 antibodyBRCC5 antibody

  DNA repair protein RAD51 homolog 1 antibodyDNA repair protein rhp51 antibodyHRAD51 antibodyHsRad51 antibodyHsT16930 antibodyRad 51 antibodyRAD51 antibodyRAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibodyRAD51 homolog A antibodyRAD51 homolog antibodyRAD51_HUMAN antibodyRAD51A antibodyRECA antibodyRecA like protein antibodyRecA, E. coli, homolog of antibodyRecombination protein A antibody

  see all

Anti-Rad51 antibody images

• 

  Western blot - Rad51 antibody (ab84304)
  All lanes : Anti-Rad51 antibody (ab84304) at 1 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
  Lane 3 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 36 kDa
  Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 20 minutes
• 

  Western blot - Anti-Rad51 antibody (ab84304)
  Anti-Rad51 antibody (ab84304) at 1 µg/ml + PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 36 kDa
  Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 55 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 12 minutes