Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
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All lanes : Anti-Rad51 (phospho T309) antibody (ab31769) at 1 µg/ml
Lane 1 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 4 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM) with
Lane 5 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate with
Lane 6 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate with
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 37 kDa
Observed band size : 39 kDa (why is the actual band size different from the predicted?)
Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
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