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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Overview
Product name
Anti-Rad51 (phospho T309) antibody
See all Rad51 products (18) ...
Description
Rabbit polyclonal to Rad51 (phospho T309)
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Rabbit, Chicken, Cow, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Rad51, phosphorylated at T309.
(Peptide available as ab33130.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: Jurkat (Staurosporine Treated 24hr, 500nM) Ramos Y79
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
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Western blot - Rad51 (phospho T309) antibody (ab31769)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 39 kDa (predicted molecular weight: 37 kDa).
Target
Function
May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
Tissue specificity
Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
Involvement in disease
Defects in RAD51 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Sequence similarities
Belongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain.
Post-translational
modifications
Phosphorylated. Phosphorylation of Thr-309 by CHEK1/CHK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination.
Cellular localization
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion matrix. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels.
Target information above from: UniProt accessionQ06609
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BRCA1/BRCA2 containing complex, subunit 5 antibody
- BRCC 5 antibody
- BRCC5 antibody
see all
Database links
- Entrez Gene: 396086 Chicken
- Entrez Gene: 514749 Cow
- Entrez Gene: 5888 Human
- Entrez Gene: 19361 Mouse
- Entrez Gene: 499870 Rat
- Omim: 179617 Human
- SwissProt: P37383 Chicken
- SwissProt: Q2KJ94 Cow
see all
Anti-Rad51 (phospho T309) antibody images:
Western blot - Rad51 (phospho T309) antibody (ab31769)
All lanes : Anti-Rad51 (phospho T309) antibody (ab31769) at 1 µg/ml
Lane 1 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 4 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM) with Rad51 peptide - phospho T309 (ab33130) at 1 µg/ml
Lane 5 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate with Rad51 peptide - phospho T309 (ab33130) at 1 µg/ml
Lane 6 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate with Rad51 peptide - phospho T309 (ab33130) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 37 kDa
Observed band size : 39 kDa (why is the actual band size different from the predicted?)
Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
References for Anti-Rad51 (phospho T309) antibody (ab31769)
ab31769 has not yet been referenced specifically in any publications.
Publishing research using ab31769? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"