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Western blot - Rad51 (phospho Y315) antibody (ab61111)
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Product Name
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Rad51 (phospho Y315) antibody
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See all Rad51 antibodies (15)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rad51 (phospho Y315)
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Immunogen
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Synthetic phosphopeptide derived from human RAD51 around the phosphorylation site of tyrosine 315 (K-I-YP-D-S).
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Reacts with
(species key)
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Hu, Ms
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Specificity
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ab61111 detects endogenous levels of RAD51 only when phosphorylated
at tyrosine 315.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
WB: 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from Jurkat cells
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
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Relevance
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Rad51 is a recombinase that is essential for homologous recombination and double-strand break repair. It binds to single and double-stranded DNA, and has DNA-dependent ATPase activity. Rad51 unwinds duplex DNA and forms helical nucleoprotein filaments in a manner that is controlled through the action of DNA helicases that counteract nucleofilament formation. Rad51 interacts with BRCA1 and BRCA2, and also plays a role in p53-mediated signalling. Interaction of Rad51 with Chk1 kinase may occur in response to Chk1 phosphorylation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad51 (phospho Y315) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rad51 (phospho Y315) antibody images, references, products related to ab61111 and other tools.
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Rad51 (phospho Y315) antibody images:
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 Western blot - Rad51 (phospho Y315) antibody (ab61111)
All lanes : Rad51 (phospho Y315) antibody (ab61111) at 1/500 dilution
Lane 1 : Extract from Jurkat cells
Lane 2 : Extract from Jurkat cells with immunising Rad51 (phospho Y315) peptide
Predicted band size : 37 kDa
Observed band size : 37 kDa
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Search PubMed (MEDLINE) for references to Rad51
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Rad51 (phospho Y315) antibody - more information
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Customer reviews (feedback) regarding Rad51 (phospho Y315) antibody |
Customer FAQs regarding Rad51 (phospho Y315) antibody |
Protocols for Rad51 (phospho Y315) antibody |
Price and availability of products related to Rad51 (phospho Y315) antibody |
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Rad51 (phospho Y315) antibody - related products:
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Rad51 (phospho Y315) antibody - other tools:
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Contact Abcam with a Technical Enquiry about Rad51 (phospho Y315) antibody |
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