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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Overview
Product name
Anti-Rad51 (phospho Y315) antibody
See all Rad51 products (18) ...
Description
Rabbit polyclonal to Rad51 (phospho Y315)
Specificity
ab61111 detects endogenous levels of RAD51 only when phosphorylated at tyrosine 315.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic phosphopeptide derived from human RAD51 around the phosphorylation site of tyrosine 315 (K-I-YP-D-S).
Positive control
Extracts from Jurkat cells
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
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Western blot - Rad51 (phospho Y315) antibody (ab61111)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/10000ELISA: 1/10000
WB: 1/500 - 1/1000.Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
Target
Function
May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
Tissue specificity
Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
Involvement in disease
Defects in RAD51 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Sequence similarities
Belongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain.
Post-translational
modifications
Phosphorylated. Phosphorylation of Thr-309 by CHEK1/CHK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination.
Cellular localization
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion matrix. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels.
Target information above from: UniProt accessionQ06609
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BRCA1/BRCA2 containing complex, subunit 5 antibody
- BRCC 5 antibody
- BRCC5 antibody
see all
Database links
- Entrez Gene: 5888 Human
- Entrez Gene: 19361 Mouse
- Omim: 179617 Human
- SwissProt: Q06609 Human
- SwissProt: Q08297 Mouse
- Unigene: 631709 Human
- Unigene: 330492 Mouse
Anti-Rad51 (phospho Y315) antibody images:
Western blot - Rad51 (phospho Y315) antibody (ab61111)
All lanes : Anti-Rad51 (phospho Y315) antibody (ab61111) at 1/500 dilution
Lane 1 : Extract from Jurkat cells
Lane 2 : Extract from Jurkat cells with immunising Rad51 (phospho Y315) peptide
Predicted band size : 37 kDa
Observed band size : 37 kDa
References for Anti-Rad51 (phospho Y315) antibody (ab61111)
ab61111 has not yet been referenced specifically in any publications.
Publishing research using ab61111? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"