Overview

  • Product nameAnti-Rad51C antibodySee all Rad51C primary antibodies ...
  • Description
    Rabbit polyclonal to Rad51C
  • Tested applicationsICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human Rad51C.

  • Positive control
    • Extracts from COS-7 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab72063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500. Use with paraformaldehyde fixed cells.
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 42 kDa).

Target

  • FunctionEssential for the homologous recombination (HR) pathway of DNA repair. Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The RAD51B-RAD51C dimer exhibits single-stranded DNA-dependent ATPase activity. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Participates in branch migration and Holliday junction resolution and thus is important for processing HR intermediates late in the DNA repair process. Also has an early function in DNA repair in facilitating phosphorylation of the checkpoint kinase CHK2 and thereby transduction of the damage signal, leading to cell cycle arrest and HR activation. Protects RAD51 from ubiquitin-mediated degradation that is enhanced following DNA damage. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and XRCC3. Contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability. Involved in maintaining centrosome number in mitosis.
  • Tissue specificityExpressed in a variety of tissues, with highest expression in testis, heart muscle, spleen and prostate.
  • Involvement in diseaseDefects in RAD51C are the cause of Fanconi anemia complementation group O (FANCO) [MIM:613390]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
    Defects in RAD51C are the cause of breast-ovarian cancer familial type 3 (BROVCA3) [MIM:613399]. It is a condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate.
  • Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. DNA damage induces an increase in nuclear levels. Accumulates in DNA damage induced nuclear foci or RAD51C foci which is formed during the S or G2 phase of cell cycle. Accumulation at DNA lesions requires the presence of NBN/NBS1, ATM and RPA.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA repair protein RAD51 homolog 3 antibody
    • MGC104277 antibody
    • R51H3 antibody
    • RA51C_HUMAN antibody
    • Rad 51C antibody
    • RAD51 homolog C (S. cerevisiae) antibody
    • Rad51 homolog c (S. cerevisiae), isoform CRA_b antibody
    • RAD51 homolog C antibody
    • RAD51 like protein 2 antibody
    • RAD51, S. cerevisiae, homolog of, C antibody
    • RAD51-like protein 2 antibody
    • RAD51C antibody
    • RAD51C protein antibody
    • RAD51L2 antibody
    • RAD51L2/RAD51C protein antibody
    • RGD1563765 antibody
    • Yeast RAD51 homolog 3 antibody
    see all

Anti-Rad51C antibody images

  • All lanes : Anti-Rad51C antibody (ab72063) at 1/500 dilution

    Lane 1 : Extracts from COS-7 cells
    Lane 2 : Extracts from COS-7 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <19 kDa. We are unsure as to the identity of these extra bands.
  • ab72063 (1/500) staining Rad51C in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

    See Abreview

References for Anti-Rad51C antibody (ab72063)

ab72063 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

Submitted Apr 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"