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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Overview
Product name
Anti-Rad51D antibody [Rad51D 5B3/6]
See all Rad51D products (4) ...
Description
Mouse monoclonal [Rad51D 5B3/6] to Rad51D
Specificity
Does not cross react with RAD51B, RAD51C, XRCC2 or XRCC3 in Westerns. Does not recognise Rad51D in HeLa total extracts.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length protein (Human) which has been his-tagged and overexpressed in E.coli.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
The antibody was purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-Page.
Clonality
Monoclonal
Clone number
Rad51D 5B3/6
Myeloma
Sp2/0-Ag14
Isotype
IgG1
Light chain type
unknown
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab20243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 35 kDa.
Target
Function
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
Tissue specificity
Expressed in colon, prostate, spleen, testis, ovary, thymus and small intestine. Weakly expressed in leukocytes.
Sequence similarities
Belongs to the RecA family. RAD51 subfamily.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO75771
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA repair protein RAD51 homolog 4 antibody
- OTTHUMP00000163851 antibody
- OTTHUMP00000163852 antibody
see all
Database links
- Entrez Gene: 5892 Human
- Omim: 602954 Human
- SwissProt: O75771 Human
- Unigene: 631757 Human
References for Anti-Rad51D antibody [Rad51D 5B3/6] (ab20243)
ab20243 has not yet been referenced specifically in any publications.
Publishing research using ab20243? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"