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Anti-Rad51D antibody (ab74036)

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Overview

Product name

Anti-Rad51D antibody
See all Rad51D products (4) ...

Description

Rabbit polyclonal to Rad51D

Specificity

This antibody detects endogenous levels of total Rad51D protein.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

A synthesized peptide derived from the internal region of human Rad51D.

Positive control

Extracts from Jurkat cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.

  • Western blot - Rad51D antibody (ab74036)Western blot - Rad51D antibody (ab74036) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab74036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Predi...Read more →

    WB: 1/500 - 1/1000.Predicted molecular weight: 35 kDa.

  • ELISA

     ELISA: 1/20000

    ELISA: 1/20000

Target

Function

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.

Tissue specificity

Expressed in colon, prostate, spleen, testis, ovary, thymus and small intestine. Weakly expressed in leukocytes.

Sequence similarities

Belongs to the RecA family. RAD51 subfamily.

Cellular localization

Nucleus.

Target information above from: UniProt accessionO75771 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DNA repair protein RAD51 homolog 4 antibody
  • OTTHUMP00000163851 antibody
  • OTTHUMP00000163852 antibody
  • OTTHUMP00000163853 antibody
  • R51H3 antibody
  • RA51D_HUMAN antibody
  • RAD51 homolog D (S. cerevisiae) antibody
  • RAD51 homolog D antibody
  • RAD51 like 3 (S. cerevisiae) antibody
  • RAD51-like protein 3 antibody
  • RAD51L3 antibody
  • Recombination repair protein antibody
  • Trad antibody
see all

Database links

Anti-Rad51D antibody images:

  Western blot - Rad51D antibody (ab74036)

Western blot - Rad51D antibody (ab74036)

All lanes : Anti-Rad51D antibody (ab74036) at 1/500 dilution

Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells plus 5µg immunizing peptide

Lysates/proteins at 5 µg per lane.


Predicted band size : 35 kDa
Observed band size : 39 kDa (why is the actual band size different from the predicted?)

References for Anti-Rad51D antibody (ab74036)

ab74036 has not yet been referenced specifically in any publications.

Publishing research using ab74036? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"