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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Anti-Rad51L1 antibody [1 H3/13] (ab3637)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab3637? Please let us know so that we can cite the reference in this datasheet
ab3637 has been referenced in 3 publications.
- Albala JS et al. Identification of a novel human RAD51 homolog, RAD51B. Genomics 46:476-9 (1997). PubMed: 9441753
- Gildemeister OS et al. Cellular redistribution of Rad51 in response to DNA damage: novel role for Rad51C. J Biol Chem 284:31945-52 (2009). WB; Human. PubMed: 19783859
- Masson JY et al. Identification and purification of two distinct complexes containing the five RAD51 paralogs. Genes Dev 15:3296-307 (2001). PubMed: 11751635
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - Rad51L1 antibody [1 H3/13] (ab3637)](/ps/datasheet/images/3/ab3637/Rad51L1-Primary-antibodies-ab3637-1.jpg)
Western blot - Rad51L1 antibody [1 H3/13] (ab3637)
Predicted band size : 42 kDa
Proteins were separated on 4–12% SDS-polyacrylamide gels and transferred onto Immun-Blot polyvinylidene difluoride membranes at 20 V for 40 minutes using a semi-dry transfer apparatus. Blots were rinsed for 5 minutes with blocking buffer (10 mm Tris-HCl (pH 8.0), 300 mm NaCl, and 0.25% Tween 20) and blocked with 15% non-fat milk in blocking buffer for 1 hour with slow rocking. Blots were incubated with primary antibody ab3637 (RAD51B) for at least 1 hour at room temperature (or overnight at 4 °C), after which they were washed with blocking buffer (3× 5 minutes) and incubated for 1 hour with horseradish peroxidase-conjugated secondary antibody. After a thorough washing with blocking buffer (6× 5 minutes), specific bands were detected by chemiluminescence on a Fuji LAS-3000 luminescent image analyzer or by exposure to x-ray film. Bands were quantified and normalized to loading controls by analyzing imported tiffs of scanned blots using ImageJ or by using the Fuji LAS-3000 software.
Image from Gildemeister OS et al, J Biol Chem. 2009 Nov 13;284(46):31945-52. Epub 2009 Sep 26, Fig S3.
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