Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
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Can you please tell me the immunogen sequence this Rad51L1 antibody was raised against? Do you have any information about which isoforms this antibody detects? |
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ANSWER: |
Thank you for contacting us. Details of the immunogen for ab3637 are considered proprietary. We do know that this antibody shows no cross-reactivity with Rad51C, Rad51D, Rad51, XRCC2, or XRCC3 in western blot analysis. I hope this helps, please let me know if you need any additional information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 42 kDa
Proteins were separated on 4–12% SDS-polyacrylamide gels and transferred onto Immun-Blot polyvinylidene difluoride membranes at 20 V for 40 minutes using a semi-dry transfer apparatus. Blots were rinsed for 5 minutes with blocking buffer (10 mm Tris-HCl (pH 8.0), 300 mm NaCl, and 0.25% Tween 20) and blocked with 15% non-fat milk in blocking buffer for 1 hour with slow rocking. Blots were incubated with primary antibody ab3637 (RAD51B) for at least 1 hour at room temperature (or overnight at 4 °C), after which they were washed with blocking buffer (3× 5 minutes) and incubated for 1 hour with horseradish peroxidase-conjugated secondary antibody. After a thorough washing with blocking buffer (6× 5 minutes), specific bands were detected by chemiluminescence on a Fuji LAS-3000 luminescent image analyzer or by exposure to x-ray film. Bands were quantified and normalized to loading controls by analyzing imported tiffs of scanned blots using ImageJ or by using the Fuji LAS-3000 software.
Image from Gildemeister OS et al, J Biol Chem. 2009 Nov 13;284(46):31945-52. Epub 2009 Sep 26, Fig S3.
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