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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Overview
Product name
Anti-Rad51L1 antibody
See all Rad51L1 products (8) ...
Description
Rabbit polyclonal to Rad51L1
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthesized peptide derived from an internal sequence of human RAD51L1.
Positive control
Human pancreas tissue; extracts from COS-7 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ELISA
ELISA: 1/40000ELISA: 1/40000
IHC-P: 1/50 - 1/100.
Target
Function
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. The RAD51B-RAD51C dimer exhibits single-stranded DNA-dependent ATPase activity. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
Tissue specificity
Expressed in a wide range of tissues.
Involvement in disease
Note=A chromosomal aberration involving RAD51L1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with HMGA1.
Note=A chromosomal aberration involving RAD51L1 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with HMGA2.
Sequence similarities
Belongs to the RecA family. RAD51 subfamily.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO15315
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA repair protein RAD51 homolog 2 antibody
- hREC2 antibody
- MGC34245 antibody
see all
Database links
- Entrez Gene: 5890 Human
- Entrez Gene: 19363 Mouse
- Omim: 602948 Human
- SwissProt: O15315 Human
- SwissProt: O35719 Mouse
- Unigene: 172587 Human
- Unigene: 341756 Mouse
Anti-Rad51L1 antibody images:
Western blot - Rad51L1 antibody (ab69622)
All lanes : Anti-Rad51L1 antibody (ab69622) at 1/500 dilution
Lane 1 : Extracts from COS-7 cells with no immunising peptide
Lane 2 : Extracts from COS-7 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 42 kDa
Observed band size : 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rad51L1 antibody (ab69622)
Immunohistochemical analysis of Rad51L1 in paraffin embedded human pancreas tissue using ab69622 at a dilution of 1/50.
References for Anti-Rad51L1 antibody (ab69622)
ab69622 has not yet been referenced specifically in any publications.
Publishing research using ab69622? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"