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Western blot - Rad51L1 antibody (ab69622)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rad51L1 antibody (ab69622)
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Product Name
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Rad51L1 antibody
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See all Rad51L1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rad51L1
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Immunogen
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Synthesized peptide derived from an internal sequence of human RAD51L1.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/40000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human pancreas tissue; extracts from COS-7 cells.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
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Relevance
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Rad51L1, also known as Rad51B, plays a role in the homologous recombination of double-stranded DNA breaks arising during DNA replication or resulting from exposure to DNA damaging agents. Rad51L1 may promote the assembly of Rad51 nucleoprotein filaments at these sites. the Rad51L1/Rad51C heterodimer exhibits single stranded DNA-dependent ATPase activity. Rad51L1 is a component of the BCDX2 complex, which also contains Rad51C, Rad51D and XRCC2. The BCDX2 complex binds to single-stranded DNA, single-stranded gaps in duplex DNA, and to nicks in duplex DNA. Overexpression of Rad51L1 causes G1 delay and apoptosis, consistent with its role in sensing DNA damage.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad51L1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rad51L1 antibody images, references, products related to ab69622 and other tools.
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Rad51L1 antibody images:
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 Western blot - Rad51L1 antibody (ab69622)
All lanes : Rad51L1 antibody (ab69622) at 1/500 dilution
Lane 1 : Extracts from COS-7 cells with no immunising peptide
Lane 2 : Extracts from COS-7 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 42 kDa
Observed band size : 42 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rad51L1 antibody (ab69622)
Immunohistochemical analysis of Rad51L1 in paraffin embedded human pancreas tissue using ab69622 at a dilution of 1/50.
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Search PubMed (MEDLINE) for references to Rad51L1
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Rad51L1 antibody - more information
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Customer reviews (feedback) regarding Rad51L1 antibody |
Customer FAQs regarding Rad51L1 antibody |
Protocols for Rad51L1 antibody |
Price and availability of products related to Rad51L1 antibody |
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Rad51L1 antibody - related products:
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Rad51L1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Rad51L1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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