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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Overview
Product name
Anti-Rad9 antibody
See all Rad9 products (9) ...
Description
Rabbit polyclonal to Rad9
Specificity
Ab50311 specifically recognizes recombinant RAD9.
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Cow, Cynomolgus Monkey
Immunogen
Synthetic peptide: PQGPSPVLAEDSEGEG corresponding to amino acids 376 - 391 of Human Rad9, conjugated to KLH by a N terminal Cysteine residue linker. The immunizing peptide corresponds to RAD9A and is not present in RAD9B.
PQGPSPVLAE DSEGEG
Positive control
WB: extracts of 293T cells transfected with RAD9.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).
Target
Function
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Sequence similarities
Belongs to the rad9 family.
Post-translational
modifications
Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ99638
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell cycle checkpoint control protein antibody
- Cell cycle checkpoint control protein RAD9A antibody
- DNA repair exonuclease rad9 homolog A antibody
see all
Database links
- Entrez Gene: 5883 Human
- Entrez Gene: 19367 Mouse
- Omim: 603761 Human
- SwissProt: Q96C41 Human
- SwissProt: Q99638 Human
- SwissProt: Q9Z0F6 Mouse
- Unigene: 655354 Human
- Unigene: 277629 Mouse
References for Anti-Rad9 antibody (ab50311)
ab50311 has not yet been referenced specifically in any publications.
Publishing research using ab50311? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"