Rad9 antibody (ab50311)

Product Name 

Rad9 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Rad9

Immunogen 

Synthetic peptide: PQGPSPVLAEDSEGEG corresponding to amino acids 376 - 391 of Human Rad9, conjugated to KLH by a N terminal Cysteine residue linker. The immunizing peptide corresponds to RAD9A and is not present in RAD9B.
BLAST 'PQGPSPVLAEDSEGEG' with or

Reacts with 

Hu

Predicted to react 
(species key)

Cow, CynMk
(Due to sequence homology)

Specificity 

Ab50311 specifically recognizes recombinant RAD9.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: extracts of 293T cells transfected with RAD9.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition

Relevance 

Rad 9 is a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.

Form 

Liquid

Concentration 

7.600 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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