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Anti-Rad9 antibody (ab59022) at 0.3 µg/ml + A431 cell lysate (35µg protein in RIPA buffer)
Predicted band size : 43 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
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