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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Overview
Product name
Anti-Rad9 antibody
See all Rad9 products (9) ...
Description
Goat polyclonal to Rad9
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Cow, Dog, Cynomolgus Monkey
Immunogen
Synthetic peptide: C-QGPSPVLAEDSEGE, corresponding to C terminal amino acids 377-391 of Human Rad9
C-QGPSPVLA EDSEGE
Positive control
A431 and Daudi cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
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Western blot - Rad9 antibody (ab59022)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
Peptide ELISA: antibody detection limit dilution 1:32,000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 43 kDa). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Volkmer et al, J Biol Chem. 1999 Jan 8;274(2):567-70.; PMID: 9872989).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Sequence similarities
Belongs to the rad9 family.
Post-translational
modifications
Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ99638
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell cycle checkpoint control protein antibody
- Cell cycle checkpoint control protein RAD9A antibody
- DNA repair exonuclease rad9 homolog A antibody
see all
Database links
- Entrez Gene: 5883 Human
- Entrez Gene: 19367 Mouse
- Omim: 603761 Human
- SwissProt: Q96C41 Human
- SwissProt: Q99638 Human
- SwissProt: Q9Z0F6 Mouse
- Unigene: 655354 Human
- Unigene: 277629 Mouse
Anti-Rad9 antibody images:
Western blot - Rad9 antibody (ab59022)
Anti-Rad9 antibody (ab59022) at 0.3 µg/ml + A431 cell lysate (35µg protein in RIPA buffer)
Predicted band size : 43 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-Rad9 antibody (ab59022)
ab59022 has not yet been referenced specifically in any publications.
Publishing research using ab59022? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"