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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Overview
Product name
Anti-Rad9 antibody
See all Rad9 products (9) ...
Description
Rabbit polyclonal to Rad9
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the C-terminal (P334) region of human Rad9, conjugated to KLH.
Positive control
Y79 cell line lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
ab63048 was purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
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Western blot - Rad9 antibody (ab63048)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100.Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Function
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Sequence similarities
Belongs to the rad9 family.
Post-translational
modifications
Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ99638
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell cycle checkpoint control protein antibody
- Cell cycle checkpoint control protein RAD9A antibody
- DNA repair exonuclease rad9 homolog A antibody
see all
Database links
- Entrez Gene: 5883 Human
- Omim: 603761 Human
- SwissProt: Q96C41 Human
- SwissProt: Q99638 Human
- Unigene: 655354 Human
Anti-Rad9 antibody images:
Western blot - Rad9 antibody (ab63048)
Anti-Rad9 antibody (ab63048) at 1/60 dilution + Y79 cell line lysates at 35 µg
Predicted band size : 43 kDa
Observed band size : 43 kDa
References for Anti-Rad9 antibody (ab63048)
ab63048 has not yet been referenced specifically in any publications.
Publishing research using ab63048? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"