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Western blot - Rad9 antibody (ab63048)
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Product Name
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Rad9 antibody
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See all Rad9 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rad9
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Immunogen
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Synthetic peptide from the C-terminal (P334) region of human Rad9, conjugated to KLH.
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Reacts with
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Hu
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Tested applications
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
WB: 1/50 - 1/100. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Y79 cell line lysates.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
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Relevance
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Rad 9 is a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab63048 was purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rad9 antibody images, references, products related to ab63048 and other tools.
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Rad9 antibody images:
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 Western blot - Rad9 antibody (ab63048)
Rad9 antibody (ab63048) at 1/60 dilution + Y79 cell line lysates at 35 µg
Predicted band size : 43 kDa
Observed band size : 43 kDa
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Search PubMed (MEDLINE) for references to Rad9
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Rad9 antibody - more information
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Customer reviews (feedback) regarding Rad9 antibody |
Customer FAQs regarding Rad9 antibody |
Protocols for Rad9 antibody |
Price and availability of products related to Rad9 antibody |
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Rad9 antibody - related products:
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Rad9 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Rad9 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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