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Thanks for your great offer. Your information is very helpful to me. Yes, I would like to proceed and test ab70810 in ChIP, since my aim is to select any RAD9 antibody whic works for chip-on-chip, is it OK for you to send me another mouse monoclonal ab117848 to me, so I can test them in parallel? I will wait for your answer and follow your suggestion for the following process. Best Regards. |
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ANSWER: |
AB70810 DISCOUNT CODE: XXXX Expiration date: AB117848 DISCOUNT CODE: XXXX Expiration date: I am very pleased to hear you would like to accept our offer and test both ab70810 and ab117848 and in ChIP. Above are the code that I have created for each testing discount. Please note the the discount code is attached to the specific antibody that you will be reviewing. These codes will give you 1 free primary antibody each before the expiration date. To redeem this offer, please submit an Abreview in ChIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. Thank you for your reply. I can send you 100% Abreview codes for both products. Each would require that you test and submit and Abreview before you could recieve the free primary antibody. |
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Hi, Good morning. I need do chip-on-chip experiment on a gene named RAD9A. I searched your website, Abcam has 9 RAD9A pimary antibdies, but none of them is a chip graded RAD9A primary antibody in Abcam list. My silly question: is there any possible that Abcam can make a chip graded RAD9A primary antibody? Best |
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ANSWER: |
Thank you for contacting us. While we do not have a ChIP graded RAD9A antibody. It is highly likely that the products we do have may work in your experiment. Ideally, specific antibodies for ChIP should be affinity-purified. Polyclonal antibodies are also recommended over monoclonal antibodies. Ab70810 Anti-Rad9 antibody is an affinity purified rabbit polyclonal that has been shown to work in IP, Western blot and IHC. If you are interested in testing this antibody in ChIP on human tissue I would be able to offer you a 100% Abreview discount. Under program I can offer a discount off a future purchase if you buy ab70810 test it in ChIP submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. Details of this offer are below. Should the rabbit polyclonal not be appropriate for your experiments, ab117848 Anti-Rad9 is an affinity purified mouse monoclonal that may also be an excellent candidate for your experiments. In that case, you the 100% Abreview discount for this product. The only caveat being that polyclonal antibodies could recognize more epitopes than a monoclonal and therefore have a greater chance of working. These are the links to the two products that I have recommended: ab70810: http://www.abcam.com/Rad9-antibody-ab70810.html ab117848: http://www.abcam.com/Rad9-antibody-7B11-ab117848.html If you have not previously validated an antibody for ChIP, I would suggest that you review section 2 of the protocol booklet I've attached: "How to validate an antibody for ChIP" If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab70810 in ChIP. I will then send a discount code. This code must be issued before purchasing ab70810 so please wait for my reply before ordering. 2. Purchase ab70810 either by phone, fax, or online (www.abcam.com). 3. Test it in ChIP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab70810 turns out to be unsuitable for ChIP you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-Rad9 antibody (ab70810) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 43 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
1mg whole cell lysate from HeLa cells was immunoprecipitated using ab70810 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70810 at 1ug/ml.
Detection: chemiluminescence with exposure time of 30 seconds.
IHC image of ab70810 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab70810, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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