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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Overview
Product name
Anti-Rad9 antibody
See all Rad9 products (9) ...
Description
Rabbit polyclonal to Rad9
Tested applications
IHC-P, WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residue 350 and the C-terminus (residue 391) of Human Rad9 (NP_004575.1)
Positive control
Whole cell lysate from HeLa and 293T cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB: 1/2000 - 1/10000.Detects a band of approximately 55 kDa (predicted molecular weight: 43 kDa).
IP: Use at 2-5 µg/mg of lysate.
Target
Function
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Sequence similarities
Belongs to the rad9 family.
Post-translational
modifications
Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ99638
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell cycle checkpoint control protein antibody
- Cell cycle checkpoint control protein RAD9A antibody
- DNA repair exonuclease rad9 homolog A antibody
see all
Database links
- Entrez Gene: 5883 Human
- Omim: 603761 Human
- SwissProt: Q96C41 Human
- SwissProt: Q99638 Human
- Unigene: 655354 Human
Anti-Rad9 antibody images:
Western blot - Rad9 antibody (ab70810)
All lanes : Anti-Rad9 antibody (ab70810) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 43 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
Immunoprecipitation - Rad9 antibody (ab70810)
1mg whole cell lysate from HeLa cells was immunoprecipitated using ab70810 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70810 at 1ug/ml.
Detection: chemiluminescence with exposure time of 30 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Rad9 antibody(ab70810)
IHC image of ab70810 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab70810, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Rad9 antibody (ab70810)
ab70810 has not yet been referenced specifically in any publications.
Publishing research using ab70810? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"