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Western blot - Rad9 antibody (ab70810)
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Immunoprecipitation - Rad9 antibody (ab70810)
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Product Name
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Rad9 antibody
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See all Rad9 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rad9
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Immunogen
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Synthetic peptide corresponding to a region between residue 350 and the C-terminus (residue 391) of Human Rad9 (NP_004575.1)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Whole cell lysate from HeLa and 293T cells.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
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Relevance
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Rad 9 is a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rad9 antibody images, references, products related to ab70810 and other tools.
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Rad9 antibody images:
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 Western blot - Rad9 antibody (ab70810)
All lanes : Rad9 antibody (ab70810) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 43 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
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Immunoprecipitation - Rad9 antibody (ab70810)
1mg whole cell lysate from HeLa cells was immunoprecipitated using ab70810 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70810 at 1ug/ml.
Detection: chemiluminescence with exposure time of 30 seconds.
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Search PubMed (MEDLINE) for references to Rad9
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Rad9 antibody - more information
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Customer reviews (feedback) regarding Rad9 antibody |
Customer FAQs regarding Rad9 antibody |
Protocols for Rad9 antibody |
Price and availability of products related to Rad9 antibody |
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Rad9 antibody - related products:
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Rad9 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Rad9 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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