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Western blot - Radixin antibody [AF1H1] (ab85323)
![Western blot - Radixin antibody [AF1H1] (ab85323)](/ps/datasheet/images/85/ab85323/Radixin-Primary-antibodies-ab85323-1.jpg)
(enlarge)
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Product Name
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Radixin antibody [AF1H1]
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See all Radixin antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [AF1H1] to Radixin
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Immunogen
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His tagged recombinant fragment, corresponding to amino acids 1-310 of Human Radixin, from E.coli.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
WB: 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A431, HeLa, HepG2, K562, 293T, PC12 and NIH3T3 cell lysates.
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Cellular localization
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Cell membrane. Cytoplasm; cytoskeleton. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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Relevance
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Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin.
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Radixin antibody [AF1H1] (ab85323) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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AF1H1
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Isotype
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IgG2b
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Light chain type
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kappa
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Purity
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Ammonium Sulphate Precipitation
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Storage buffer
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Preservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Radixin antibody [AF1H1] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Radixin antibody [AF1H1] images, references, products related to ab85323 and other tools.
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Radixin antibody [AF1H1] images:
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 Western blot - Radixin antibody [AF1H1] (ab85323)
All lanes : Radixin antibody [AF1H1] (ab85323) at 1/10000 dilution
Lane 1 : A431 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : HepG2 cell lysates
Lane 4 : K562 cell lysates
Lane 5 : 293T cell lysates
Lane 6 : PC12 cell lysates
Lane 7 : NIH3T3 cell lysates
Predicted band size : 69 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to Radixin
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Radixin antibody [AF1H1] - more information
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Customer reviews (feedback) regarding Radixin antibody [AF1H1] |
Customer FAQs regarding Radixin antibody [AF1H1] |
Protocols for Radixin antibody [AF1H1] |
Price and availability of products related to Radixin antibody [AF1H1] |
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Radixin antibody [AF1H1] - related products:
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Radixin antibody [AF1H1] - other tools:
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Contact Abcam with a Technical Enquiry about Radixin antibody [AF1H1] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - Radixin antibody [AF1H1] (ab85323)](/index.html?datasheet=85323#Radixin-Primary-antibodies-ab85323-1.jpg)
