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Western blot - Radixin antibody [EP1862Y] (ab52495)
![Western blot - Radixin antibody [EP1862Y] (ab52495)](/ps/datasheet/images/52495 WB.jpg)
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Immunohistochemistry (Paraffin-embedded sections) - Radixin antibody [EP1862Y] (ab52495)
![Immunohistochemistry (Paraffin-embedded sections) - Radixin antibody [EP1862Y] (ab52495)](/ps/datasheet/images/52495 IHC.jpg)
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Product Name
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Radixin antibody [EP1862Y]
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See all Radixin antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EP1862Y] to Radixin
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Immunogen
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Synthetic peptide corresponding to residues near the N terminus of human Radixin
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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Flow Cyt, ICC, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/35.
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250 (An antigen retrieval step is recommended)
IP: 1/100.
WB: 1/20,000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: HeLa cell lysate
IHC-P: Paraffin embedded human ovarian carcinoma
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Cellular localization
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Cell membrane. Cytoplasm; cytoskeleton. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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Relevance
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Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EP1862Y
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Radixin antibody [EP1862Y] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Radixin antibody [EP1862Y] images, references, products related to ab52495 and other tools.
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Radixin antibody [EP1862Y] images:
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 Western blot - Radixin antibody [EP1862Y] (ab52495)
Radixin antibody [EP1862Y] (ab52495) at 1/20000 dilution + HeLa cell lysate at 10 µg
Secondary
HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size : 80 kDa
Observed band size : 80 kDa
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Immunohistochemistry (Paraffin-embedded sections) - Radixin antibody [EP1862Y] (ab52495)
Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using ab52495 at 1/100 dilution.
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Search PubMed (MEDLINE) for references to Radixin
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Radixin antibody [EP1862Y] - more information
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Customer reviews (feedback) regarding Radixin antibody [EP1862Y] |
Customer FAQs regarding Radixin antibody [EP1862Y] |
Protocols for Radixin antibody [EP1862Y] |
Price and availability of products related to Radixin antibody [EP1862Y] |
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Radixin antibody [EP1862Y] - related products:
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Radixin antibody [EP1862Y] - other tools:
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Contact Abcam with a Technical Enquiry about Radixin antibody [EP1862Y] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - Radixin antibody [EP1862Y] (ab52495)](/index.html?datasheet=52495#52495 WB.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - Radixin antibody [EP1862Y] (ab52495)](/index.html?datasheet=52495#52495 IHC.jpg)
