Anti-Radixin antibody [EP1862Y] (ab52495)

  Western blot - Radixin antibody [EP1862Y] (ab52495)

Anti-Radixin antibody [EP1862Y] (ab52495) at 1/20000 dilution + HeLa cell lysate at 10 µg

Secondary
HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size : 80 kDa
Observed band size : 80 kDa

  Immunohistochemistry (Paraffin-embedded sections) - Radixin antibody [EP1862Y] (ab52495)

Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using ab52495 at 1/100 dilution.

  Immunocytochemistry/ Immunofluorescence-Radixin antibody [EP1862Y](ab52495)

ICC/IF image of ab52495 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52495, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Flow Cytometry - Radixin antibody [EP1862Y] (ab52495)

Overlay histogram showing HeLa cells stained with ab52495 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52495, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.