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Anti-Radixin antibody (ab50238)

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Overview

Product name

Anti-Radixin antibody
See all Radixin products (8) ...

Description

Rabbit polyclonal to Radixin

Tested applications

WB, IP, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Predicted to work with

Pig

Immunogen

Synthetic peptide: KSAIAKQAAD conjugated to KLH, corresponding to internal sequence amino acids 400-409, with an N-terminal added tripeptide, of Human RDX

KSAIAKQAAD

Positive control

Whole extract of cultured mouse NIH-3T3 cells, whole extract of cultured human acute T cell leukemia Jurkat cells, HeLa cells or extract or rat liver.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Applications

Show applications key

Our Abpromise guarantee covers the use of ab50238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     
  • IP

     
  • ICC/IF

     

Application notes

Indirect ICC/IF: Use at a concentration of 10 - 20 µg/ml, staining cultured human HeLa cells.

IP: Use 10-20µg to immunoprecipitate Radixin from 250ug RIPA of rat liver. Following use of the antibody in immunoprecipitation, neither ezrin nor moesin are
detectable by their respective specific antibodies.

WB: Use at a concentration of 1-2µg/ml using a whole extract of cultured mouse NIH-3T3 cells and a chemiluminescence detection reagent. Use at a concentration of 0.5-1ug/ml using a whole extract of cultured human acute T cell leukemia Jurkat cells and a chemiluminescence detection reagent. Predicted molecular weight: 69 kDa.

Additional weak bands may be detected when immunoblotting some extract preparations. Detection of RDX by immunoblotting is specifically inhibited with the immunizing peptide.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.

Involvement in disease

Defects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Sequence similarities

Contains 1 FERM domain.

Domain

The N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ablilty to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN.

Post-translational
modifications

Phosphorylated by tyrosine-protein kinases.

Cellular localization

Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.

Target information above from: UniProt accessionP35241 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CB567 antibody
  • CG12537 antibody
  • DFNB24 antibody
  • ESP10 antibody
  • Hh-induced MATH and BTB domain-containing protein antibody
  • HIB antibody
  • Moesin-B antibody
  • Protein roadkill antibody
  • RADI_HUMAN antibody
  • Radixin antibody
  • RDX antibody
see all

References for Anti-Radixin antibody (ab50238)

ab50238 has not yet been referenced specifically in any publications.

Publishing research using ab50238? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"