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Product Name
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Radixin antibody
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See all Radixin antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Radixin
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Immunogen
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Synthetic peptide: KSAIAKQAAD conjugated to KLH, corresponding to internal sequence amino acids 400-409, with an N-terminal added tripeptide, of Human RDX
BLAST 'KSAIAKQAAD' with  or 
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Pig
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Indirect ICC/IF: Use at a concentration of 10 - 20 µg/ml, staining cultured human HeLa cells.
IP: Use 10-20µg to immunoprecipitate Radixin from 250ug RIPA of rat liver. Following use of the antibody in immunoprecipitation, neither ezrin nor moesin are
detectable by their respective specific antibodies.
WB: Use at a concentration of 1-2µg/ml using a whole extract of cultured mouse NIH-3T3 cells and a chemiluminescence detection reagent. Use at a concentration of 0.5-1ug/ml using a whole extract of cultured human acute T cell leukemia Jurkat cells and a chemiluminescence detection reagent. Predicted molecular weight: 69 kDa.
Additional weak bands may be detected when immunoblotting some extract preparations. Detection of RDX by immunoblotting is specifically inhibited with the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Whole extract of cultured mouse NIH-3T3 cells, whole extract of cultured human acute T cell leukemia Jurkat cells, HeLa cells or extract or rat liver.
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Cellular localization
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Cell membrane. Cytoplasm; cytoskeleton. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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Relevance
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Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin.
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Radixin antibody (ab50238) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.80 - 0.90 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Radixin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Radixin
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Radixin antibody - more information
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Customer reviews (feedback) regarding Radixin antibody |
Customer FAQs regarding Radixin antibody |
Protocols for Radixin antibody |
Price and availability of products related to Radixin antibody |
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Contact Abcam with a Technical Enquiry about Radixin antibody |
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