Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Overview
Product name
Anti-Radixin antibody
See all Radixin products (8) ...
Description
Rabbit polyclonal to Radixin
Tested applications
WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Pig
Immunogen
Synthetic peptide: KSAIAKQAAD conjugated to KLH, corresponding to internal sequence amino acids 400-409, with an N-terminal added tripeptide, of Human RDX
KSAIAKQAAD
Positive control
Whole extract of cultured mouse NIH-3T3 cells, whole extract of cultured human acute T cell leukemia Jurkat cells, HeLa cells or extract or rat liver.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IP
ICC/IF
Application notes
Indirect ICC/IF: Use at a concentration of 10 - 20 µg/ml, staining cultured human HeLa cells.
IP: Use 10-20µg to immunoprecipitate Radixin from 250ug RIPA of rat liver. Following use of the antibody in immunoprecipitation, neither ezrin nor moesin are
detectable by their respective specific antibodies.
WB: Use at a concentration of 1-2µg/ml using a whole extract of cultured mouse NIH-3T3 cells and a chemiluminescence detection reagent. Use at a concentration of 0.5-1ug/ml using a whole extract of cultured human acute T cell leukemia Jurkat cells and a chemiluminescence detection reagent. Predicted molecular weight: 69 kDa.
Additional weak bands may be detected when immunoblotting some extract preparations. Detection of RDX by immunoblotting is specifically inhibited with the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
Involvement in disease
Defects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Sequence similarities
Contains 1 FERM domain.
Domain
The N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ablilty to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN.
Post-translational
modifications
Phosphorylated by tyrosine-protein kinases.
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
Target information above from: UniProt accessionP35241
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CB567 antibody
- CG12537 antibody
- DFNB24 antibody
see all
Database links
- Entrez Gene: 5962 Human
- Entrez Gene: 19684 Mouse
- Entrez Gene: 494457 Pig
- Entrez Gene: 315655 Rat
- Omim: 179410 Human
- SwissProt: P35241 Human
- SwissProt: P26043 Mouse
- SwissProt: P26044 Pig
see all
References for Anti-Radixin antibody (ab50238)
ab50238 has not yet been referenced specifically in any publications.
Publishing research using ab50238? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"