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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Overview
Product name
Anti-Radixin antibody
See all Radixin products (8) ...
Description
Goat polyclonal to Radixin
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Pig, Zebrafish
Immunogen
Peptide (aa 537-546) with sequence C-QARDETKKTQND, from the internal region of the protein sequence (Human)
QARDETKKTQ ND
Positive control
Human Tonsil; Lymph Node and Spleen lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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Western blot - Radixin antibody (ab51982)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/4000ELISA: 1/4000
WB: Use a concentration of 1 - 3 µg/ml.Detects a band of approximately 80 kDa.
Target
Function
Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
Involvement in disease
Defects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Sequence similarities
Contains 1 FERM domain.
Domain
The N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ablilty to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN.
Post-translational
modifications
Phosphorylated by tyrosine-protein kinases.
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
Target information above from: UniProt accessionP35241
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CB567 antibody
- CG12537 antibody
- DFNB24 antibody
see all
Database links
- Entrez Gene: 395511 Chicken
- Entrez Gene: 517111 Cow
- Entrez Gene: 479446 Dog
- Entrez Gene: 5962 Human
- Entrez Gene: 19684 Mouse
- Entrez Gene: 494457 Pig
- Entrez Gene: 315655 Rat
- Entrez Gene: 286739 Zebrafish
see all
Anti-Radixin antibody images:
Western blot - Radixin antibody (ab51982)
Anti-Radixin antibody (ab51982) at 0.03 µg/ml + Human Tonsil lysate in RIPA buffer at 35 µg
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-Radixin antibody (ab51982)
ab51982 has not yet been referenced specifically in any publications.
Publishing research using ab51982? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"