Feedback Form

Western blot - Radixin antibody (ab51982)
Western blot - Radixin antibody (ab51982)
(enlarge)


Product Name 

Radixin antibody

 

See all Radixin antibodies (5)...

Product type 

Primary antibodies

Description 

Goat polyclonal to Radixin

Immunogen 

Peptide (aa 537-546) with sequence C-QARDETKKTQND, from the internal region of the protein sequence (Human)
BLAST 'QARDETKKTQND' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat, Chk, Cow, Dog, Pig, Zfsh
(Due to sequence homology)

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 220 Abpoints.


Application notes 
(see key)

Recommended dilutions
ELISA: 1/4000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 80 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human Tonsil; Lymph Node and Spleen lysates

Cellular localization 

Cell membrane. Cytoplasm; cytoskeleton. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins

Relevance 

Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin.

 

Alternative names Radixin antibody (ab51982)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

395511    (Chicken)
517111    (Cow)
5962    (Human)
19684    (Mouse)
494457    (Pig)
315655    (Rat)
286739    (Zebrafish)

Omim

    

179410    (Human)

SwissProt

    

Q9PU45    (Chicken)
Q32LP2    (Cow)
P35241    (Human)
P26043    (Mouse)
P26044    (Pig)

Unigene

    

592679    (Human)
263671    (Human)
203261    (Rat)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Radixin antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Radixin antibody images, references, products related to ab51982 and other tools.

 

Radixin antibody images:

Product image  Western blot - Radixin antibody (ab51982)

Western blot - Radixin antibody (ab51982)

Radixin antibody (ab51982) at 0.03 µg/ml + Human Tonsil lysate in RIPA buffer at 35 µg

Observed band size : 80 kDa (why is the actual band size different from the predicted?)


Primary incubation was 1 hour. Detected by chemiluminescence.

 
 

Search PubMed (MEDLINE) for references to Radixin

 

Radixin antibody - more information

Customer reviews (feedback) regarding Radixin antibody

Customer FAQs regarding Radixin antibody

Protocols for Radixin antibody

Price and availability of products related to Radixin antibody

 

Radixin antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for Radixin antibody

ab29686

  

Spleen (Human) Cytoplasmic Lysate - adult normal tissue

ab29687

  

Spleen (Human) Cytoplasmic Lysate - fetal normal tissue

ab29689

  

Spleen (Human) Membrane Lysate - adult normal tissue

ab29862

  

Lymph node (Human) Cytoplasmic Lysate - adult normal tissue

ab29865

  

Lymph node (Human) Membrane Lysate - adult normal tissue

 

Compatible Secondaries for Radixin antibody

ab6566

  

Cy5 ® antibody

ab6739

  

Texas Red ® antibody

ab6740

  

Biotin antibody

ab6741

  

HRP antibody

ab6742

  

Alkaline Phosphatase antibody

ab6881

  

FITC antibody

ab7004

  

Phycoerythrin antibody

ab27245

  

10nm Gold antibody

ab27246

  

10nm Gold antibody

ab27247

  

15nm Gold antibody

 

Other Radixin Antibodies

See all Radixin antibodies (5)...

 

Radixin antibody - other tools:

Contact Abcam with a Technical Enquiry about Radixin antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.