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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-Raf1 antibody [410]
See all Raf1 products (27) ...
Description
Mouse monoclonal [410] to Raf1
Specificity
This antibody is specifically reactive to Raf-1 N-terminal (1-240 a.a.)
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Purified recombinant human Raf-1 (mw:75 kDa).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration
Concentration information loading...
Purification notes
Affinity purified from ascitic fluid.
Clonality
Monoclonal
Clone number
410
Myeloma
unknown
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cell Biology >> Apoptosis >> Mitochondrial
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
This antibody, at a concentration of 1.0 µg/ml, will allow visualization of 0.2 µg/lane of human Raf-1.
Target
Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
Tissue specificity
In skeletal muscle, isoform 1 is more abundant than isoform 2.
Involvement in disease
Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity.
Cellular localization
Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes.
Target information above from: UniProt accessionP04049
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cRaf antibody
- c Raf antibody
- C-raf antibody
see all
Database links
- Entrez Gene: 5894 Human
- Omim: 164760 Human
- SwissProt: P04049 Human
- Unigene: 159130 Human
References for Anti-Raf1 antibody [410] (ab656)
ab656 has not yet been referenced specifically in any publications.
Publishing research using ab656? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"