![Western blot - Raf1 antibody [Y198] (ab32025) image](#ab32025_1.JPG)
![Immunohistochemistry (Paraffin-embedded sections) - Raf1 antibody [Y198] (ab32025) image](#ab32025_2.JPG)
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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-Raf1 antibody [Y198]
See all Raf1 products (27) ...
Description
Rabbit monoclonal [Y198] to Raf1
Tested applications
IP, IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues surrounding Serine 338-339 of human Raf1
Positive control
NIH 3T3 cell lysate and human colon carcinoma
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
Y198
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cell Biology >> Apoptosis >> Mitochondrial
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/50. Fix with 2% paraformaldehyde and permeabilize with 0.1% triton-x 100.
IHC-P: 1/50 - 1/100.
IP: 1/100.
WB: 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa).
Is unsuitable for Flow Cyt.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
Tissue specificity
In skeletal muscle, isoform 1 is more abundant than isoform 2.
Involvement in disease
Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity.
Cellular localization
Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes.
Target information above from: UniProt accessionP04049
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cRaf antibody
- c Raf antibody
- C-raf antibody
see all
Database links
- Entrez Gene: 5894 Human
- Entrez Gene: 110157 Mouse
- Entrez Gene: 24703 Rat
- Omim: 164760 Human
- SwissProt: P04049 Human
- SwissProt: Q99N57 Mouse
- SwissProt: P11345 Rat
- Unigene: 159130 Human
see all
Anti-Raf1 antibody [Y198] images:
Western blot - Raf1 antibody [Y198] (ab32025)
![Western blot - Raf1 antibody [Y198] (ab32025)](/ps/datasheet/Images/32/ab32025/ab32025_1.JPG)
Anti-Raf1 antibody [Y198] (ab32025) at 1/500 dilution + NIH 3T3 cell lysate
Predicted band size : 73 kDa
Observed band size : 74 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - Raf1 antibody [Y198] (ab32025)
![Immunohistochemistry (Paraffin-embedded sections) - Raf1 antibody [Y198] (ab32025)](/ps/datasheet/Images/32/ab32025/ab32025_2.JPG)
ab32025, at a 1/100 dilution, staining human Raf1 in colon carcinoma by Immunohistochemistry, Paraffin embedded tissue
References for Anti-Raf1 antibody [Y198] (ab32025)
This product has been referenced in:
- Dahiya Net al. Claudin-7 is frequently overexpressed in ovarian cancer and promotes invasion. PLoS One 6:e22119 (2011). WB; Human.Read more (PubMed: 21789222) »
See 1 publication for this product
Publishing research using ab32025? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"