Raf1 antibody [Y198] (ab32025)

Product Name 

Raf1 antibody [Y198]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [Y198] to Raf1

Immunogen 

Synthetic peptide corresponding to residues surrounding Serine 338-339 of human Raf1

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/50. Fix with 2% paraformaldehyde and permeabilize with 0.1% triton-x 100.
IHC-P: 1/50 - 1/100.
IP: 1/100.
WB: 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa).

Is unsuitable for Flow Cyt.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

NIH 3T3 cell lysate and human colon carcinoma

Cellular localization 

Mitochondrial outer membrane

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cell Biology >> Apoptosis >> Mitochondrial
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases

Relevance 

The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

Y198

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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