Raf1 (phospho S259) antibody (ab30621)

Product Name 

Raf1 (phospho S259) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Raf1 (phospho S259)

Immunogen 

Synthetic phosphopeptide derived from human Raf1 around the phosphorylation site of Serine 259.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Ab30621 recognises endogenous levels of Raf1 only when phosphorylated at Serine 259.

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Predicted molecular weight: 73 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

IHC-P: breast carcinoma.
WB: MDA-MB-435 cells, or K562 cells.

Cellular localization 

Mitochondrial outer membrane

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cell Biology >> Apoptosis >> Mitochondrial
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases

Relevance 

The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide.
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4.

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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