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- Secondary antibodies
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Product name
Anti-Raf1 (phospho S301) antibody
See all Raf1 products (27) ...
Description
Rabbit polyclonal to Raf1 (phospho S301)
Specificity
Specific for phospho S301 Raf1. Does not recognise non-phosphorylated S301 Raf1.
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Xenopus laevis
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho S301 of rat Raf1.
Positive control
Jurkat cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES. pH 7.5
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit serum by sequential phospho- and non-phosphopeptide affinity columns.
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cell Biology >> Apoptosis >> Mitochondrial
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
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Western blot - Raf1 (phospho S301) antibody (ab30570)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa).
Target
Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
Tissue specificity
In skeletal muscle, isoform 1 is more abundant than isoform 2.
Involvement in disease
Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity.
Cellular localization
Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes.
Target information above from: UniProt accessionP04049
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cRaf antibody
- c Raf antibody
- C-raf antibody
see all
Database links
- Entrez Gene: 5894 Human
- Entrez Gene: 110157 Mouse
- Entrez Gene: 24703 Rat
- Omim: 164760 Human
- SwissProt: P04049 Human
- SwissProt: Q99N57 Mouse
- SwissProt: P11345 Rat
- SwissProt: P09560 Xenopus laevis
see all
Anti-Raf1 (phospho S301) antibody images:
Western blot - Raf1 (phospho S301) antibody (ab30570)
Predicted band size : 73 kDa
Observed band size : 74 kDa (why is the actual band size different from the predicted?)
All lanes: UV treated human Jurkat cell lysate; ab30570 at 1/1000 dilution
Lane 1: Shows specific immunolabeling of phospho S301 Raf-1 (control).
Lane 2: Identical to lane 1, except treated with λ phosphatase (1200 units for 30 min) before incubation with ab30570
- shows immunolabeling of Raf-1 completely eliminated by treatment with λ phosphatase.
References for Anti-Raf1 (phospho S301) antibody (ab30570)
ab30570 has not yet been referenced specifically in any publications.
Publishing research using ab30570? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"