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Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate - tumor cell line (ab30124)

Q&A (4)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferpH: 7.90
    Constituents: HEPES, Potassium chloride, Sucrose, Tergitol-NP40, 100% Loading buffer
  • Concentration information loading...
  • Lysate notes Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.
  • Research Areas
  • BackgroundEstablished in 1963 from the left maxilla of a 12-year-old African boy with Burkitt's lymphoma, it is the first continuous human hematopoietic cell line. It has become an indispensable tool in haematological research and leukaemia and lymphoma studies.

    • Applications

    Our Abpromise guarantee covers the use of ab30124 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    References for Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate - tumor cell line (ab30124)

    ab30124 has not yet been referenced specifically in any publications.

    Publishing research using ab30124 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab30124

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    10
    Votes: Highest First

    Would the following product be expected to have functional proteases
    (assuming there are not denaturants in the extraction steps):
    Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate - tumor
    cell line (ab30124)

    Thank you for contacting us.


    This protein lysate could contain proteases. That’s why we put protease inhibitor cocktail in the protein lyses buffer.


    I hope this information is helpful to you. Please do not hesitate t...

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    vielen Dank für Ihre schnelle Antwort. Wir werden den von Ihnen empfohlenen Isotyp bestellen.
    Im Rahmen der Antikörperbestellung möchten wir auch eine Positivkontrolle für den Westernblot (in Form eines Lysats) mitbestellen.
    ...

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    Vielen Dank für Ihre Anfrage.

    Für ab111915 wurden 20 µg Lysat aufgetragen, für ab66106 waren es10 µg (siehe Datenblatt). Normalerweise sind - je nach Expressionslevel in der Positivkontrolle - 20 bis maximal 50 µ...

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    Dear technical support team: The customer raised an enquiry about ab30124, ab30125, ab30126 this customer wants to know which one is more suitable and could be a positive control of ab52610 (Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y])? According to th...

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    Thank you for your enquiry regarding ab52610.

    The phosphorylation of PLCG 2 at Tyrosine 1217 needs to be induced to be able to detect this specific modification. As the WB image on the datasheet indicates Raji cell lysate without stimulation wil...

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    We are trying to develop a teaching lab in which students would purify monoclonal antibodies from a cell line, and then verify/quantify their purification by ELISA.   The hybridoma that we have is L243, (produces anti-human MHC class 2), so we are now loo...

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    Thank you for contacting Abcam Our protein lysate is called total protein lysate, which means most of the proteins, including membrane and surface proteins, are within the lysate. We don’t really have a cell number to generate 100ug lysate, we can only es...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"