Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-Ran antibody [ARAN1]
See all Ran products (9) ...
Description
Mouse monoclonal [ARAN1] to Ran
Tested applications
WB, IP, ELISA, ICCmore details
Cross reactivity
Reacts with
Mouse, Hamster, Cow, Human, Xenopus laevis
Immunogen
Denatured recombinant protein (Human).
Epitope
The antibody epitope is located at the C-terminus (amino acids 207-216), which is highly negatively charged.
Positive control
Jurkat cells extract.
General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
This antibody is purified from culture supernatant of hybridoma cells, grown in a bioreactor.
Clonality
Monoclonal
Clone number
ARAN1
Isotype
IgG2b
Research areas
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Protein Trafficking >> Nuclear Import / Export
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml.Predicted molecular weight: 25 kDa.
IP: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
Target
Function
GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
Tissue specificity
Expressed in a variety of tissues.
Sequence similarities
Belongs to the small GTPase superfamily. Ran family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP62826
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Androgen receptor associated protein 24 antibody
- Androgen receptor-associated protein 24 antibody
- ARA 24 antibody
see all
Database links
- Entrez Gene: 540457 Cow
- Entrez Gene: 5901 Human
- Entrez Gene: 100045999 Mouse
- Entrez Gene: 19384 Mouse
- Entrez Gene: 100189578 Xenopus laevis
- Entrez Gene: 379874 Xenopus laevis
- GenBank: NM_006325 Human
- Omim: 601179 Human
see all
References for Anti-Ran antibody [ARAN1] (ab13049)
ab13049 has not yet been referenced specifically in any publications.
Publishing research using ab13049? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"