Ran antibody [ARAN1] (ab13049)

Product Name 

Ran antibody [ARAN1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [ARAN1] to Ran

Immunogen 

Denatured recombinant protein (Human).

Reacts with 
(species key)

Hu, Ms, Cow, Hm, Xl

Epitope 

The antibody epitope is located at the C-terminus (amino acids 207-216), which is highly negatively charged.

Tested applications 
(see key)

ELISA, ICC, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 25 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat cells extract.

Cellular localization 

Nucleus. Cytoplasm. Melanosome. Note: Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Research areas 

Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Protein Trafficking >> Nuclear Import / Export
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Cell Biology >> Cell Cycle >> Cell Division >> Spindle

Relevance 

Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in Ran disrupt DNA synthesis. Because of its many functions, it is likely that Ran interacts with several other proteins. Ran regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. Ran could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of Ran-GTP around chromatin which, in turn, induces the local nucleation of microtubules. Ran is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). Ran coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

ARAN1

Isotype 

IgG2b

Purity 

Protein A purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody is purified from culture supernatant of hybridoma cells, grown in a bioreactor.

Form 

Liquid

Concentration 

2.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Customer reviews (feedback) regarding Ran antibody [ARAN1]

Customer FAQs regarding Ran antibody [ARAN1]

Protocols for Ran antibody [ARAN1]

Price and availability of products related to Ran antibody [ARAN1]

Ran antibody [ARAN1] - related products: