|
|
|
|
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Ran antibody (ab31118)
(enlarge)
|
|
|
|
|
Product Name
|
Ran antibody
|
| |
See all Ran antibodies (8)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to Ran
|
|
Immunogen
|
Synthetic peptide: EHDLEVAQTTALPDEDDDL, corresponding to amino acids 197-216 of Human Ran
BLAST 'EHDLEVAQTTALPDEDDDL' with  or 
|
|
Reacts with
|
Hu
|
|
Predicted to react
(species key)
|
Ms, Rat
(Due to sequence homology)
|
|
Tested applications
(see key)
|
IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IHC-P: 1/50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB: Use at an assay dependent dilution. Predicted molecular weight: 24 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
HeLa and A431 cells. Normal breast
|
|
Cellular localization
|
Nucleus. Cytoplasm. Melanosome. Note: Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Protein Trafficking >> Nuclear Import / Export
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
|
|
Relevance
|
Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in Ran disrupt DNA synthesis. Because of its many functions, it is likely that Ran interacts with several other proteins. Ran regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. Ran could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of Ran-GTP around chromatin which, in turn, induces the local nucleation of microtubules. Ran is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). Ran coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.200 mg/ml
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Ran antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Ran antibody images, references, products related to ab31118 and other tools.
|
| |
Ran antibody images:
|
 Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Ran antibody (ab31118)
Human breast stained with ab31118 at a 1/50 dilution
|
| |
References for Ran antibody (ab31118)
|
|
This product has been used in: (two most recent references)
Graham JD et al. DNA replication licensing and progenitor numbers are increased by progesterone in normal human breast. Endocrinology 150:3318-26 (2009). IHC-P; Human. PubMed: 19342456
Sepp KJ et al. Identification of neural outgrowth genes using genome-wide RNAi. PLoS Genet 4:e1000111 (2008). ICC/IF; Mouse. PubMed: 18604272
If you publish research using ab31118 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to Ran
|
| |
Ran antibody - more information
|
Customer reviews (feedback) regarding Ran antibody |
Customer FAQs regarding Ran antibody |
Protocols for Ran antibody |
Price and availability of products related to Ran antibody |
| |
Ran antibody - related products:
|
|
|
| |
Ran antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Ran antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
