Anti-Ran antibody (ab4781)

Western blot - Ran antibody (ab4781)

Predicted band size : 26 kDa


ab4781 at a 1/500 dilution staining approximately 26kDa Ran in Xenopus egg extract (lane X, 50µg per lane) and HeLa total cell extract (lane Hu, 50µg per lane) by western blot (ECL, 1 minute exposure).

Immunocytochemistry/ Immunofluorescence

The top panel shows paraformaldehyde fixed HeLa cells stained with ab2254 (1/1000) and counterstained with DAPI (red). Staining with ab2254 is shown in green. In the lower panel the staining with ab2254 is quenched by the addition of the blocking peptide, ab13569.

Kirk McManus, University of British Columbia

Western blot - Ran antibody (ab4781)

Anti-Ran antibody (ab4781) at 1/500 dilution + Lysate prepared from human HUH-7 cells at 15000 cells

Secondary
HRP conjugated sheep polyclonal to rabbit IgG at 1/20000 dilution

Performed under reducing conditions.

Predicted band size : 26 kDa
Observed band size : 26 kDa


Exposure time : 3 minutes

This image is a courtesy of Anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Ran antibody(ab4781)

IHC image of ab4781 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4781, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.