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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-Ran antibody
See all Ran products (9) ...
Description
Rabbit polyclonal to Ran
Tested applications
IHC-P, IF, IP, WBmore details
Cross reactivity
Reacts with
Human, Xenopus laevis
Immunogen
Recombinant full length protein (Human) - His-tagged and purified from E. coli.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, Whole Serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Protein Trafficking >> Nuclear Import / Export
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/500Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Target
Function
GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
Tissue specificity
Expressed in a variety of tissues.
Sequence similarities
Belongs to the small GTPase superfamily. Ran family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP62826
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Androgen receptor associated protein 24 antibody
- Androgen receptor-associated protein 24 antibody
- ARA 24 antibody
see all
Database links
- Entrez Gene: 5901 Human
- Entrez Gene: 100189578 Xenopus laevis
- Entrez Gene: 379874 Xenopus laevis
- GenBank: NM_006325 Human
- Omim: 601179 Human
- SwissProt: P62826 Human
- SwissProt: P52301 Xenopus laevis
- Unigene: 10842 Human
see all
Anti-Ran antibody images:
Western blot - Ran antibody (ab4781)
Predicted band size : 26 kDa
ab4781 at a 1/500 dilution staining approximately 26kDa Ran in Xenopus egg extract (lane X, 50µg per lane) and HeLa total cell extract (lane Hu, 50µg per lane) by western blot (ECL, 1 minute exposure).
Immunocytochemistry/ Immunofluorescence
The top panel shows paraformaldehyde fixed HeLa cells stained with ab2254 (1/1000) and counterstained with DAPI (red). Staining with ab2254 is shown in green. In the lower panel the staining with ab2254 is quenched by the addition of the blocking peptide, ab13569.
Kirk McManus, University of British Columbia
Western blot - Ran antibody (ab4781)
Anti-Ran antibody (ab4781) at 1/500 dilution + Lysate prepared from human HUH-7 cells at 15000 cells
Secondary
HRP conjugated sheep polyclonal to rabbit IgG at 1/20000 dilution
Performed under reducing conditions.
Predicted band size : 26 kDa
Observed band size : 26 kDa
Exposure time : 3 minutes
This image is a courtesy of Anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Ran antibody(ab4781)
IHC image of ab4781 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4781, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Ran antibody (ab4781)
This product has been referenced in:
- Nilsen Tet al. A nuclear export sequence located on a beta-strand in fibroblast growth factor-1. J Biol Chem 282:26245-56 (2007).Read more (PubMed: 17616529) »
- Ramos Cet al. Transmembrane proteins are not required for early stages of nuclear envelope assembly. Biochem J 400:393-400 (2006).Read more (PubMed: 16953799) »
See all 2 publications for this product
Publishing research using ab4781? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"